| Fluorescent microparticles for sensing cell microenvironment oxygen levels within 3D scaffolds. | |
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MedLine Citation:
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PMID: 19285719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present the development and characterization of fluorescent oxygen-sensing microparticles designed for measuring oxygen concentration in microenvironments existing within standard cell culture and transparent three-dimensional (3D) cell scaffolds. The microparticle synthesis employs poly(dimethylsiloxane) to encapsulate silica gel particles bound with an oxygen-sensitive luminophore as well as a reference or normalization fluorophore that is insensitive to oxygen. We developed a rapid, automated and non-invasive sensor analysis method based on fluorescence microscopy to measure oxygen concentration in a hydrogel scaffold. We demonstrate that the microparticles are non-cytotoxic and that their response is comparable to that of a traditional dissolved oxygen meter. Microparticle size (5-40 microm) was selected for microscale-mapping of oxygen concentration to allow measurements local to individual cells. Two methods of calibration were evaluated and revealed that the sensor system enables characterization of a range of hypoxic to hyperoxic conditions relevant to cell and tissue biology (i.e., pO(2) 10-160 mmHg). The calibration analysis also revealed that the microparticles have a high fraction of quenched luminophore (0.90+/-0.02), indicating that the reported approach provides significant advantages for sensor performance. This study thus reports a versatile oxygen-sensing technology that enables future correlations of local oxygen concentration with individual cell response in cultured engineered tissues. |
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Authors:
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Miguel A Acosta; Patrick Ymele-Leki; Yordan V Kostov; Jennie B Leach |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-03-14 |
Journal Detail:
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Title: Biomaterials Volume: 30 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-06-04 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 3068-74 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, 1000 Hilltop Circle, ECS 314, Baltimore, MD 21250, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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chemistry Biosensing Techniques / instrumentation*, methods Calibration Cell Culture Techniques / instrumentation* Cells, Cultured Dimethylpolysiloxanes / chemistry Fluorescent Dyes / metabolism Microscopy, Fluorescence Microspheres* Oxygen / analysis*, metabolism Particle Size Tissue Engineering Tissue Scaffolds* |
| Grant Support | |
ID/Acronym/Agency:
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R01 NS065205-01A1/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Dimethylpolysiloxanes; 0/Fluorescent Dyes; 63148-62-9/baysilon; 7782-44-7/Oxygen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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