Document Detail


Fluorescent measurements of intracellular free calcium in isolated toad urinary bladder epithelial cells.
MedLine Citation:
PMID:  3112407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sodium-calcium exchange has been suggested to play a pivotal role in the regulation of cytosolic free calcium (Caf) by epithelial cells. Using isolated epithelial cells from the toad urinary bladder, Caf has been measured using the intracellular Ca-sensitive fluorescent dyes Fura 2 and Quin 2. Dye loading did not alter cell viability as assessed by measurements of ATP and ADP content or cell oxygen consumption. When basal Caf was examined over a wide range of cell dye content (from 0.04 to 180 nmol dye/mg protein) an inverse relationship was observed. At low dye content, Caf was 300-380 nM and, as dye content was increased, Caf progressively fell to 60 nM. Using low dye content cells, in which minimal alteration in Ca steady state would be expected, the role for plasma membrane Na-Ca exchange was examined using either medium sodium substitution or ouabain. While medium sodium substitution increased Caf, prolonged treatment with ouabain had no effect on Caf despite a clear increase in cell sodium content. The lack of effect of ouabain suggests that Na-Ca exchange-mediated Ca efflux plays a minimal role in the regulation of basal Caf. However, exchange-mediated Ca efflux may play a role in Caf regulation when cytosolic calcium is elevated.
Authors:
W R Jacobs; L J Mandel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  97     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  1987  
Date Detail:
Created Date:  1987-09-11     Completed Date:  1987-09-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  53-62     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bufo marinus
Calcimycin / pharmacology
Calcium / analysis*
Cyanides / pharmacology
Epithelium / drug effects,  physiology
Fluorescent Dyes
Models, Biological
Ouabain / pharmacology
Oxygen Consumption
Urinary Bladder / physiology*
Grant Support
ID/Acronym/Agency:
AM07623/AM/NIADDK NIH HHS; AM28616/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Cyanides; 0/Fluorescent Dyes; 52665-69-7/Calcimycin; 630-60-4/Ouabain; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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