Document Detail


Fluorescent location of ornithine decarboxylase employing derivatives of the specific inhibitor alpha-difluoromethyl ornithine.
MedLine Citation:
PMID:  2489234     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two fluorescent derivatives have been made from alpha-difluoromethyl ornithine by linking the carboxyl group of the ornithine derivatives to fluorescent amines. alpha-difluoromethyl ornithine is a potent inhibitor of ornithine decarboxylase, an enzyme which plays an essential role in cell division. We have used these fluorescent derivatives as probes for ornithine decarboxylase in frozen sections of skin to locate the epithelial cells which are known to contain ornithine decarboxylase. The probes also located squamous cell carcinoma cells in human skin.
Authors:
F S Steven; L A Williams; P Warne; D F Tucker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of enzyme inhibition     Volume:  3     ISSN:  8755-5093     ISO Abbreviation:  J. Enzym. Inhib.     Publication Date:  1989  
Date Detail:
Created Date:  1991-05-29     Completed Date:  1991-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709734     Medline TA:  J Enzyme Inhib     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  133-43     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Manchester, U.K.
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive
Carcinoma, Squamous Cell / enzymology*,  pathology
Eflornithine / analogs & derivatives*,  diagnostic use*,  pharmacology
Fluorescent Dyes
Humans
Mice
Microscopy, Fluorescence
Ornithine Decarboxylase / analysis*,  antagonists & inhibitors
Skin / cytology,  enzymology*
Skin Neoplasms / enzymology*,  pathology
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 70052-12-9/Eflornithine; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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