Document Detail

Fluorescent Resonance Energy Transfer Imaging of VEGFR Dimerization.
MedLine Citation:
PMID:  24778014     Owner:  NLM     Status:  In-Data-Review    
Activation of vascular endothelial growth factor receptor 1 (VEGFR1/FLT1) and 2 (VEGFR2/KDR) involves receptor dimerization. Formation of VEGFR dimer has so far not been visualized in single intact cells. In the present study we describe different optical assays which can be used to observe dimerization of VEGFR1 and VEGFR2. Bimolecular fluorescence complementation (BIFC) assays confirmed homo,- and heterodimerization of transfected receptors. Fluorescence resonance energy transfer (FRET) techniques in living and fixed CHO-K1 cells allowed observation of VEGFR1 homodimer,- and VEGFR1 and VEGFR2 heterodimer formation after ligand stimulation. After inhibition of ligand binding by the VEGFA JH121 antibody VEGFR1 homodimerization was completely abolished. Under the same conditions, cells transfected by VEGFR1 and VEGFR2 maintained relevant receptor heterodimerization. These techniques to monitor VEGFR1 and VEGFR2 homo- and heterodimerization in living and fixed cells may help in the search for new angiogenesis-directed inhibitors of VEGFR dimerization.
Zhala Ahmadova; Vugar Yagublu; Tassilo Förg; Yegana Hajiyeva; Ralf Jesenofsky; Mathias Hafner; Michael Keese
Related Documents :
24985894 - Neuropeptide y-stimulated [(35) s]gtpγs functional binding is reduced in the hippocampu...
23110884 - The effects of elevated endogenous gaba levels on movement-related network oscillations.
23793844 - Smad1 stabilization and delocalization in response to the blockade of bmp activity.
21187124 - Atp differentially upregulates fibroblast growth factor 2 and transforming growth facto...
8395534 - Early induction of ldl receptor gene during rat liver regeneration.
19490024 - Metabotropic glutamate receptor 1 activity generates persistent, n-methyl-d-aspartate r...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  34     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-04-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2123-33     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Neamine Inhibits Oral Cancer Progression by Suppressing Angiogenin-mediated Angiogenesis and Cancer ...
Next Document:  Reproducibility of Histogram and Texture Parameters Derived from Intravoxel Incoherent Motion Diffus...