Document Detail


Fluorescent C bands of human chromosomes with 33 258 Hoechst stain.
MedLine Citation:
PMID:  67076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Air-dried preparations of human metaphase chromosomes normally exhibit a Q band fluorescence pattern with 33 258 Hoechst stain while the C band regions of 1,9,16, and most acrocentric short arms appear dull. If these stained and mounted slides are stored at room temperature in the dark for several days, a spontaneous change from C-negative to C-positive band some times occurs. We postulate that the pH of the buffered saline mounting medium during storage of the slides causes the C band shift since the results can be duplicated experimentally by lowering the pH of the mounting buffer from 5.5 to 4.0.
Authors:
T R Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  36     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1977 May 
Date Detail:
Created Date:  1977-06-22     Completed Date:  1977-06-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  283-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Buffers
Cell Line
Chromosomes*
Female
Fluorescence
Humans
Hydrogen-Ion Concentration
Staining and Labeling*
Temperature
Time Factors
Chemical
Reg. No./Substance:
0/Buffers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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