Document Detail


Fluorescence spectroscopy as a tool for monitoring solubility and aggregation behavior of beta-lactoglobulin after heat treatment.
MedLine Citation:
PMID:  16767779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Denaturation and aggregation of whey proteins are of interest to the food and pharmaceutical industry due to the importance of final structure in functionality, impact on food texture, and the chemical stability of the final product. In this study, we demonstrate the potential of fluorescence spectrometry combined with multivariate chemometric methods for quantifying solubility and aggregation behavior of beta-lactoglobulin (beta-LG); a major whey protein and a frequent food ingredient. Heat-induced aggregation of beta-LG was studied under different conditions including pH, temperature and heating durations. Results showed very good agreement between the fluorescence-based predictions and measurements obtained by HPLC and gravimetric analysis regardless of the conditions. Standard normal variate (SNV), a signal preprocessing and filtering tool, was found to enhance the predictive accuracy and robustness of the fluorescence-based model.
Authors:
Rand Elshereef; Hector Budman; Christine Moresoli; Raymond L Legge
Related Documents :
14669879 - Radio frequency heating of foods: principles, applications and related properties--a re...
15726969 - Removal of pseudomonas putida biofilm and associated extracellular polymeric substances...
19906499 - The health and technological implications of a better control of neoformed contaminants...
17629299 - Myelin proteolipid protein (plp) as a marker antigen of central nervous system contamin...
21806869 - Socio-economic status and urbanization are linked to snacks and obesity in adolescents ...
8187889 - Is thermotolerance of yeast dependent on trehalose accumulation?
25023899 - The natural history of chronic urticaria in childhood: a prospective study.
23910779 - Happy eating: the single target implicit association test predicts overeating after pos...
16484529 - Hydrolysis of zeaxanthin esters by carboxyl ester lipase during digestion facilitates m...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  95     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-10-31     Completed Date:  2006-12-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  863-74     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley Periodicals, Inc.
Affiliation:
Department of Chemical Engineering, University of Waterloo, Waterloo, Ont., Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Food Technology / methods
Lactoglobulins / chemistry*
Models, Chemical*
Solubility
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Lactoglobulins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and ...
Next Document:  Numerical simulation of a fully baffled biological reactor: the differential circumferential averagi...