| Fluorescence spectroscopic study of the interaction of adenine and nucleotide with trichosanthin. | |
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MedLine Citation:
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PMID: 8528152 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trichosanthin (TCS) is an N-glycosidase that can attack the 28s rRNA of the ribosome at a highly conserved adenine residue. The interactions of adenine and its derivative nucleotides with TCS are reported. The fluorescence of Trp 192 of TCS is sensitive to the proximity of adenine, and produces a marked red shift indicative of trytophan in a more hydrophilic environment. By contrast AMP and ATP quench the maximal emission at 328nm. The binding of the adenine and ATP with TCS result in lower tryptophan accessibility to the quencher acrylamide, but higher tryptophan accessibility to the quencher iodide, while AMP caused higher tryptophan accessibility to acrylamide, and lower tryptophan accessibility to iodide. Also, the binding of nucleotides induces tryptophan heterogeneity in the protein. These findings lead us to propose that binding of nucleotides and adenine base cause different microenvironmental changes of the tryptophan residue, and Trp 192 may be involved in the active site of TCS. |
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Authors:
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Q Hao; Y Zhang; H Yang; G Liu; Z Huang; B Liu; Q Yao; Q Li |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry and molecular biology international Volume: 36 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1995 Jul |
Date Detail:
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Created Date: 1996-01-29 Completed Date: 1996-01-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 889-95 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Jilin University, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine
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chemistry*,
metabolism Adenosine Monophosphate / chemistry Adenosine Triphosphate / chemistry Antiviral Agents / chemistry, metabolism Base Sequence Conserved Sequence Glycoside Hydrolases / chemistry, metabolism Protein Conformation RNA, Ribosomal, 28S / metabolism Spectrometry, Fluorescence / methods Trichosanthin / chemistry*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antiviral Agents; 0/RNA, Ribosomal, 28S; 56-65-5/Adenosine Triphosphate; 60318-52-7/Trichosanthin; 61-19-8/Adenosine Monophosphate; 73-24-5/Adenine; EC 3.2.1.-/Glycoside Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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