| Fluorescence spectral studies on the specific interaction between sulfated glycosaminoglycans and potato lectin. | |
| | |
MedLine Citation:
|
PMID: 6874664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
On excitation at 280 nm, Solanum tuberosum agglutinin (STA) produced a fluorescence emission spectrum with a maximum at 347 nm, which is typical of tryptophanyl residues. The fluorescence emission maximum was shifted toward shorter wavelength and quenched by the addition of specific sugars. The changes were analyzed precisely and quantitatively by measuring the fluorescence difference spectra. This method required far smaller amounts of samples than UV-difference spectroscopy to obtain the binding parameters. The binding constants of chitin sulfate, keratan sulfate, and highly sulfated keratan sulfate were calculated to be 1.8 X 10(5) M-1, 3.4 X 10(5) M-1, and 5.2 X 10(5) M-1, respectively. The results indicate that consecutive and non-consecutive, alternating (1 leads to 4)-N-acetyl-beta-D-glucosaminyl residues in the glycosaminoglycans can interact freely with STA in spite of the existence of the sulfate groups at C-6 of the N-acetyl-D-glucosaminyl residues and extra sulfate groups at C-6 of the galactosyl residues linked with N-acetyl-6-O-sulfo-beta-D-glucosamine. |
| | |
Authors:
|
A Doi; I Matsumoto; N Seno |
Related Documents
:
|
1390674 - Kinetics of basic fibroblast growth factor binding to its receptor and heparan sulfate ... 21596384 - Avidin/pss membrane microcapsules with biotin-binding activity. 9927414 - The structure and function of a foot-and-mouth disease virus-oligosaccharide receptor c... 8144574 - Hepatocyte growth factor specifically binds to sulfoglycolipids. 16547024 - Nucleotide-dependent dimerization of the c-terminal domain of the abc transporter cvab ... 15814104 - The subunit composition and pharmacology of alpha-conotoxin mii-binding nicotinic acety... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of biochemistry Volume: 93 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1983 Mar |
Date Detail:
|
Created Date: 1983-09-09 Completed Date: 1983-09-09 Revised Date: 2007-12-19 |
Medline Journal Info:
|
Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
|
Languages: eng Pagination: 771-5 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Glycosaminoglycans
/
analysis* Keratan Sulfate / analysis Lectins / analysis* Spectrometry, Fluorescence Sulfates / analysis* |
| Chemical | |
Reg. No./Substance:
|
0/Glycosaminoglycans; 0/Lectins; 0/Sulfates; 9056-36-4/Keratan Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunochemical and spectral studies on Vicia faba agglutinin.
Next Document: Effect of trichloroacetic acid on the isolation of tropomyosin from sea urchin lantern muscle.