Document Detail


Fluorescence response of human HER2+ cancer- and MCF-12F normal cells to 200MHz ultrasound microbeam stimulation: a preliminary study of membrane permeability variation.
MedLine Citation:
PMID:  22513260     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Targeted mechanical cell stimulation has been extensively studied for a better understanding of its effect on cellular mechanotransduction signaling pathways and structures by utilizing a variety of mechanical sources. In this work, an ultrasound-driven single cell stimulation method is thus proposed, and a preliminary study is carried out by comparing the fluorescence intensities representing a change in cell membrane permeability between MDA-MB-435 human HER2+ cancer cells (∼40-50μm in diameter) and MCF-12F normal cells (∼50-60μm) in the presence of ultrasound. A 200MHz single element zinc oxide (ZnO) transducer is employed to generate ultrasound microbeam (UM) whose beamwidth and depth of focus are 9.5 and 60μm, comparable to typical cell size. The cells in tetramethyl rhodamine methyl ester (TMRM) are interrogated with 200MHz sinusoidal bursts. The number of cycles per burst is 5 and the pulse repetition frequency (PRF) is 1kHz. The temporal variation of fluorescence intensity in each cell is measured as a function of input voltage to the transducer (16, 32, and 47V), and its corresponding fluorescence images are obtained via a confocal microscope. A systematic method for visualizing UM's focus by adding Rhodamine B to the immersion medium is also proposed to enhance the precision in aiming the beam at an individual cell. Both types of cells exhibit a decrease in the intensity upon UM irradiation. In particular, normal cells show more fluorescence reduction (down to 0.7 in normalized intensity) than cancer cells (∼0.9) under the same excitation condition of the transducer. With UM being turned off, the normalized intensity level in normal cells is slowly increased to 1.1. The cell images taken before and after UM exposure indicate that the intensity reduction is more pronounced in those cells after exposure. Hence the results show the potential of UM as a non-invasive in vitro stimulation tool for facilitating targeted drug delivery and gene transfection as well as for studying cellular mechanotransduction.
Authors:
Jae Youn Hwang; Jungwoo Lee; Changyang Lee; Anette Jakob; Robert Lemor; Lali K Medina-Kauwe; K Kirk Shung
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-29
Journal Detail:
Title:  Ultrasonics     Volume:  52     ISSN:  1874-9968     ISO Abbreviation:  Ultrasonics     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-09     Completed Date:  2012-10-24     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0050452     Medline TA:  Ultrasonics     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  803-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Membrane Permeability
Feasibility Studies
Fluorescence
Humans
Mechanotransduction, Cellular*
Receptor, erbB-2 / metabolism*
Rhodamines
Transducers
Ultrasonics / methods*
Zinc Oxide
Grant Support
ID/Acronym/Agency:
P41 EB002182/EB/NIBIB NIH HHS; P41-EB2182/EB/NIBIB NIH HHS; R01 EB012058/EB/NIBIB NIH HHS; R01 EB012058-01A1/EB/NIBIB NIH HHS; R01-CA129822/CA/NCI NIH HHS; R01-CA140995/CA/NCI NIH HHS; R01-EB012058/EB/NIBIB NIH HHS; R21-CA116014/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Rhodamines; 0/tetramethylrhodamine methyl ester; EC 2.7.10.1/Receptor, erbB-2; SOI2LOH54Z/Zinc Oxide
Comments/Corrections

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