| Fluorescence nanoscopy by ground-state depletion and single-molecule return. | |
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MedLine Citation:
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PMID: 18794861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach. |
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Authors:
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Jonas Fölling; Mariano Bossi; Hannes Bock; Rebecca Medda; Christian A Wurm; Birka Hein; Stefan Jakobs; Christian Eggeling; Stefan W Hell |
Publication Detail:
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Type: Journal Article Date: 2008-09-15 |
Journal Detail:
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Title: Nature methods Volume: 5 ISSN: 1548-7105 ISO Abbreviation: Nat. Methods Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-31 Completed Date: 2008-11-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101215604 Medline TA: Nat Methods Country: United States |
Other Details:
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Languages: eng Pagination: 943-5 Citation Subset: IM |
Affiliation:
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Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Cell Survival Humans Microscopy, Fluorescence / methods* Spectrometry, Fluorescence / methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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