Document Detail


Fluorescence microscopy-based RNA interference screening.
MedLine Citation:
PMID:  25391794     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Using RNAi interference (RNAi), it is possible to study the effect of specific gene knockdowns in mammalian cells. In this protocol we present the automated preparation of "ready to transfect" multiwell plates and cell arrays, on which cells can be grown which are then reversely transfected with one type of siRNA in every individual well or spot. Additionally, different microscope types for screening approaches are compared and considerations about the information workflow are made.
Authors:
Manuel Gunkel; Nina Beil; Jürgen Beneke; Jürgen Reymann; Holger Erfle
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1251     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2015  
Date Detail:
Created Date:  2014-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-66     Citation Subset:  IM    
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