Document Detail


Fluorescence line-narrowing spectrometry: a versatile tool for the study of chemically initiated carcinogenesis.
MedLine Citation:
PMID:  2128812     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An important initiating step in the induction of tumors is believed to be the covalent binding of an active carcinogenic species to a cellular macromolecule, e.g. DNA. Therefore, a spectroscopic technique which allows for positive identification of the intact (macromolecular) DNA adduct and/or isolated damaged nucleosides/nucleotides is highly desirable. It is shown that fluorescence line-narrowing spectroscopy (FLNS) is a rapid, versatile, highly sensitive and selective analytical technique, which can be used directly to characterize DNA adducts and isolated nucleosides. FLNS possesses sufficient resolution to distinguish between the major DNA adducts derived from different enantiomers of benzo[a]pyrene diol-epoxide (BPDE). With the present limit of detection (approximately 1 adducted base per 10(8) normal base pairs for 100 micrograms of DNA), the technique is applicable to in vivo samples. Analysis of liver DNA from fish exposed to benzo[a]pyrene (BP) (100 mg BP/kg fish) showed that a major DNA adduct is derived from syn-BPDE.
Authors:
R Jankowiak; P Lu; G J Small; M Nishimoto; U Varanasi; S K Kim; N E Geacintov
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  8     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  1990  
Date Detail:
Created Date:  1991-07-03     Completed Date:  1991-07-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  113-21     Citation Subset:  IM    
Affiliation:
Ames Laboratory-USDOE, Iowa State University 50011.
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MeSH Terms
Descriptor/Qualifier:
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / analysis*,  metabolism*
Carcinogens / metabolism*
DNA / analysis*,  metabolism*
DNA Adducts*
Spectrometry, Fluorescence
Stereoisomerism
Grant Support
ID/Acronym/Agency:
CA 20851/CA/NCI NIH HHS; Y01-CP-40507/CP/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Carcinogens; 0/DNA Adducts; 0/benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; 55097-80-8/7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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