| Fluorescence in situ hybridization techniques for cytogenetic and genomic analyses. | |
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MedLine Citation:
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PMID: 23135841 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Fluorescent in situ hybridization (FISH) is a powerful method to visualize DNA sequences in the context of the whole chromosome. Yet despite the value of FISH analysis for cytogenetic studies, there are surprisingly few labs that are able to adapt the technique for their experiments in chromosomal and genome biology. Here we present a comprehensive FISH protocol acquired from over 20 years of collective experience using different plant species. Our description uses rice as a model for performing a complete FISH procedure, but the protocol can be readily adapted for other plant species. We have provided more specialized instruction beyond routine FISH, which includes the preparation of meiotic and mitotic samples suitable for FISH analysis, procedures for direct and indirect labeling of DNA probes, and techniques for increasing signal strength using layers of antibodies. |
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Authors:
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Jason G Walling; Wenli Zhang; Jiming Jiang |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 956 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 13-27 Citation Subset: IM |
Affiliation:
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Department of Horticulture, University of Wisconsin-Madison, Madison, WI, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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