Document Detail

Fluorescence in situ hybridization analysis of the fos/jun ratio in the ageing brain.
MedLine Citation:
PMID:  10197789     Owner:  NLM     Status:  MEDLINE    
We have examined the expression of the fos and jun genes in the cerebellum of the rat brain during ageing, by use of a semi-quantitative fluorescence in situ hybridization (FISH) method. In these experiments we have utilised the digital imaging capabilities of a cooled CCD camera system to store the fluorescence intensities of individual cells and to compare the data from each target (fos or jun) gene with that of a control (beta-actin) gene. In this way we have been able to obtain a relative quantitation of fos and jun mRNA levels. Purkinje cells were analysed in brain from Sprague-Dawley rats of 6, 13 and 23 months of age. Data obtained in this way demonstrated that the level of fos expression decreased significantly during ageing but, in contrast, that of jun increased between 6 and 13 months and thereafter remained constant. We subsequently carried out a further comparison of fos/jun ratios in purkinje cells in Wistar rats and also observed a highly significant fall in the ratio between 6 and 23 months. This change in the fos/jun ratio has important implications for the composition of the AP-1 transcription factor and for the expression of genes that it regulates.
M Salehi; M Barron; B J Merry; M H Goyns
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  107     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-06-01     Completed Date:  1999-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  61-71     Citation Subset:  IM    
School of Health Sciences, University of Sunderland, UK.
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MeSH Terms
Aging / genetics*
Cerebellum / cytology,  metabolism*
Gene Expression
Genes, fos / genetics
Genes, jun / genetics
In Situ Hybridization, Fluorescence
Proto-Oncogene Proteins c-fos / metabolism*
Proto-Oncogene Proteins c-jun / metabolism*
Purkinje Cells / cytology,  metabolism
RNA, Messenger / biosynthesis
Rats, Sprague-Dawley
Rats, Wistar
Reproducibility of Results
Species Specificity
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger

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