Document Detail

Fluorescence of hematoporphyrin in living cells and in solution.
MedLine Citation:
PMID:  3149989     Owner:  NLM     Status:  MEDLINE    
The fluorescence properties of hematoporphyrin (Hp) and its derivative (HpD) were investigated in leukemia cells and in normal lymphocytes under a microscope, and the results were compared with those in solution. The spectra and the time behaviour of Hp (or HpD) fluorescence in living cells were found to be almost the same as those in Hp solution of very high concentration. This implies that Hp is much more concentrated in the cells than in the medium. It was also found that irradiation with intense light easily gives rise to a photoproduct which gives an additional peak in the fluorescence spectrum. Possible methods to increase the sensitivity of cancer detection and localization are discussed.
S Kinoshita; T Seki; T F Liu; T Kushida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  2     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1989-06-16     Completed Date:  1989-06-16     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  195-208     Citation Subset:  IM    
Department of Physics, Osaka University, Japan.
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MeSH Terms
Hematoporphyrins / physiology*
Leukocytes / physiology
Lymphocytes / physiology
Spectrometry, Fluorescence
Reg. No./Substance:

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