| Fluorescence of hematoporphyrin in living cells and in solution. | |
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MedLine Citation:
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PMID: 3149989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The fluorescence properties of hematoporphyrin (Hp) and its derivative (HpD) were investigated in leukemia cells and in normal lymphocytes under a microscope, and the results were compared with those in solution. The spectra and the time behaviour of Hp (or HpD) fluorescence in living cells were found to be almost the same as those in Hp solution of very high concentration. This implies that Hp is much more concentrated in the cells than in the medium. It was also found that irradiation with intense light easily gives rise to a photoproduct which gives an additional peak in the fluorescence spectrum. Possible methods to increase the sensitivity of cancer detection and localization are discussed. |
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Authors:
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S Kinoshita; T Seki; T F Liu; T Kushida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of photochemistry and photobiology. B, Biology Volume: 2 ISSN: 1011-1344 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1989-06-16 Completed Date: 1989-06-16 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 195-208 Citation Subset: IM |
Affiliation:
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Department of Physics, Osaka University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescence* Hematoporphyrins / physiology* Leukocytes / physiology Lymphocytes / physiology Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Hematoporphyrins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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