Document Detail


Fluorescence of hematoporphyrin in living cells and in solution.
MedLine Citation:
PMID:  3149989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fluorescence properties of hematoporphyrin (Hp) and its derivative (HpD) were investigated in leukemia cells and in normal lymphocytes under a microscope, and the results were compared with those in solution. The spectra and the time behaviour of Hp (or HpD) fluorescence in living cells were found to be almost the same as those in Hp solution of very high concentration. This implies that Hp is much more concentrated in the cells than in the medium. It was also found that irradiation with intense light easily gives rise to a photoproduct which gives an additional peak in the fluorescence spectrum. Possible methods to increase the sensitivity of cancer detection and localization are discussed.
Authors:
S Kinoshita; T Seki; T F Liu; T Kushida
Related Documents :
18315499 - A one-day, dispense-only ip-one htrf assay for high-throughput screening of galphaq pro...
7511129 - Characterization and enrichment of fetal rat hepatoblasts by immunoadsorption ("panning...
7763479 - Selection of defined cell types by flow-cytometric cell sorting.
7894239 - Cellular effects of image diagnostic ultrasound on murine spermatogenesis monitored by ...
20004969 - Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell d...
11157219 - Gfp-based n-acyl homoserine-lactone sensor systems for detection of bacterial communica...
1557029 - Inhibition of cell proliferation in saccharomyces cerevisiae by expression of human nad...
9883879 - Glycan engineering of proteins with whole living yeast cells expressing rat liver alpha...
8136159 - Cell cycle-dependent expression of cyclin d1 and a 45 kd protein in human a549 lung car...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  2     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1989-06-16     Completed Date:  1989-06-16     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  195-208     Citation Subset:  IM    
Affiliation:
Department of Physics, Osaka University, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Fluorescence*
Hematoporphyrins / physiology*
Leukocytes / physiology
Lymphocytes / physiology
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Hematoporphyrins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The dose-response relationship for tumourigenesis by UV radiation in the region 311-312 nm.
Next Document:  Characterization of new furocoumarin derivatives by their dark and light-mediated action on RNA bact...