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Fluorescence fluctuation spectroscopy approaches to the study of receptors in live cells.
MedLine Citation:
PMID:  23280108     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Communication between cells and their environment, including other cells, is often mediated by cell surface receptors. Fluorescence methodologies are among the most important techniques used to study receptors and their interactions, and in the past decade, fluorescence fluctuation spectroscopy (FFS) approaches have been increasingly utilized. In this overview, we illustrate how diverse FFS approaches have been used to elucidate important aspects of receptor systems, including interactions of receptors with their ligands and receptor oligomerization and clustering. We also describe the most popular methods used to introduce fluorescent moieties into the biological systems. Finally, specific attention will be given to cell maintenance and transfection strategies especially as related to microscopy studies.
Authors:
David M Jameson; Nicholas G James; Joseph P Albanesi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  519     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-113     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
Affiliation:
Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA. Electronic address: djameson@hawaii.edu.
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