| Fluorescence fluctuation spectroscopy approaches to the study of receptors in live cells. | |
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MedLine Citation:
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PMID: 23280108 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Communication between cells and their environment, including other cells, is often mediated by cell surface receptors. Fluorescence methodologies are among the most important techniques used to study receptors and their interactions, and in the past decade, fluorescence fluctuation spectroscopy (FFS) approaches have been increasingly utilized. In this overview, we illustrate how diverse FFS approaches have been used to elucidate important aspects of receptor systems, including interactions of receptors with their ligands and receptor oligomerization and clustering. We also describe the most popular methods used to introduce fluorescent moieties into the biological systems. Finally, specific attention will be given to cell maintenance and transfection strategies especially as related to microscopy studies. |
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Authors:
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David M Jameson; Nicholas G James; Joseph P Albanesi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 519 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 87-113 Citation Subset: IM |
Copyright Information:
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Copyright © 2013 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA. Electronic address: djameson@hawaii.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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