| Fluorescence enhancement of yttrium(III)-rutin by nucleic acids in the presence of cetyltrimethylammonium bromide. | |
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MedLine Citation:
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PMID: 16644263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is found that nucleic acids can enhance the fluorescence intensity of yttrium(III) (Y(3+))-rutin in presence of cetyltrimethylammonium bromide (CTMAB) system. In hexamethylenetetramine (HMTA)-HCl buffer, the maximum enhanced fluorescence is produced, with maximum excitation and emission wavelengths at 452 and 520 nm, respectively. Based on this, a new fluorimetric method of determination of nucleic acids is proposed. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentration of nucleic acids in the range of 1.0 x 10(-7) to 1.0 x 10(-5)g/ml for fish sperm DNA (fsDNA), 1.0 x 10(-7) to 4.6 x 10(-6)g/ml for yeast RNA (yRNA), their detection limits (S/N=3) are 7.5 x 10(-8), 8.0 x 10(-8)g/ml, respectively. The interaction mechanism is also studied. |
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Authors:
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Xia Wu; Changying Guo; Fei Wang; Jinghe Yang; Dehuan Ran; Jinhua Zheng; Jinbo Wu |
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9138893 - Quantification of nucleic acids. 12005353 - Cytotoxicity issues pertinent to lipoplex-mediated gene therapy in-vivo. 15329333 - Amino acids composition of teucrium nutlet proteins and their systematic significance. |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-27 |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 65 ISSN: 1386-1425 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-10 Completed Date: 2007-02-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
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Languages: eng Pagination: 543-8 Citation Subset: IM |
Affiliation:
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Key Laboratory of Colloid and Interface Chemistry, Shandong University, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cetrimonium Compounds / chemistry* DNA / analysis* Fishes / genetics Fluorescence Male RNA / analysis* RNA, Fungal / analysis Rutin / chemistry* Spectrometry, Fluorescence / methods* Spermatozoa / chemistry Yeasts / genetics Yttrium / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Cetrimonium Compounds; 0/RNA, Fungal; 153-18-4/Rutin; 63231-63-0/RNA; 6899-10-1/cetrimonium; 7440-65-5/Yttrium; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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