| Fluorescence enhancement effect of morin-nucleic acid-L-cysteine-capped nano-ZnS system and the determination of nucleic acid. | |
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MedLine Citation:
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PMID: 18575642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is found that L-cysteine-capped nano-ZnS can further enhance the fluorescence intensity of the morin-nucleic acid system. Under optimum conditions, the enhanced intensity of fluorescence is proportional to the concentration of nucleic acid in the range of 7.0 x 10(-8)-1.0 x 10(-5) g mL(-1) for fish sperm DNA (fsDNA) and 9.0 x 10(-8)-5.0 x 10(-6) g mL(-1) for yeast RNA (yRNA). The corresponding detection limits (S/N = 3) are 2.0 x 10(-8) g mL(-1) and 4.0 x 10(-8) g mL(-1), respectively. The interaction mechanisms of morin-nucleic acid-L-cysteine-capped nano-ZnS system are studied by multiple techniques. It is considered that there exists synergistic effects of groove binding and electrostatic interaction between morin, L-cysteine-capped nano-ZnS and nucleic acid, and the complex of morin-L-cysteine-capped nano-ZnS-nucleic acid is formed. |
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Authors:
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Liangliang Zhao; Xia Wu; Honghong Ding; Jinghe Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-03 |
Journal Detail:
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Title: The Analyst Volume: 133 ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-25 Completed Date: 2008-09-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
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Languages: eng Pagination: 896-902 Citation Subset: IM |
Affiliation:
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A Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, Shandong, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cysteine Flavonoids Hydrogen-Ion Concentration Male Nanostructures* Nucleic Acids / analysis* Spectrometry, Fluorescence / instrumentation, methods Spermatozoa / ultrastructure Sulfides Zinc Compounds |
| Chemical | |
Reg. No./Substance:
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0/Flavonoids; 0/Nucleic Acids; 0/Sulfides; 0/Zinc Compounds; 1314-98-3/zinc sulfide; 480-16-0/morin; 52-90-4/Cysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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