Document Detail


Fluorescence emission properties of 8-aza analogues of caffeine, theophylline, and related N-alkyl xanthines.
MedLine Citation:
PMID:  19250833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescence emission properties of 8-azacaffeine, 8-azatheophylline and other N-alkylated 8-azaxanthines (8-azaXan) have been examined. It is shown that N-methylated 8-azaxanthines, as well as 8-azatheophylline, are highly fluorescent in aqueous medium as the neutral, and, in some instances, also as the monoanionic, forms. 8-Azacaffeine exhibits moderate emission, but its isomer, 1,3,8-trimethyl-8-azaXan, is highly fluorescent. All three 8-azaxanthines monomethylated on the triazole ring, as well as 8-azaxanthosine, exhibit increased acidity in the excited state. Some fluorescent pyrazolo[4,3-d]pyrimidine-5,7-diones, xanthine congeners of pyrazolo[4,3-d]pyrimidines, are also reported. Many of these are good fluorescent probes in enzymatic, receptor binding, and nucleic acid systems, some examples of which are presented. In particular, 8-azaXan is an excellent fluorescent probe for purine nucleoside phosphorylases, as a fluorogenic substrate in the reverse, synthetic pathway.
Authors:
Grzegorz Medza; Jacek Wierzchowski; Borys Kierdaszuk; David Shugar
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Publication Detail:
Type:  Journal Article     Date:  2009-01-14
Journal Detail:
Title:  Bioorganic & medicinal chemistry     Volume:  17     ISSN:  1464-3391     ISO Abbreviation:  Bioorg. Med. Chem.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-20     Completed Date:  2009-06-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9413298     Medline TA:  Bioorg Med Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  2585-91     Citation Subset:  IM    
Affiliation:
Department of Biophysics, University of Warmia and Mazury, 4 Oczapowskiego Street, PL-10-719 Olsztyn, Poland.
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MeSH Terms
Descriptor/Qualifier:
Aza Compounds / chemistry*
Caffeine / analogs & derivatives*,  chemistry
Spectrometry, Fluorescence / methods*
Spectrophotometry, Ultraviolet
Theophylline / analogs & derivatives*,  chemistry
Xanthines / chemistry*
Chemical
Reg. No./Substance:
0/Aza Compounds; 0/Xanthines; 58-08-2/Caffeine; 58-55-9/Theophylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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