| Fluorescence correlation spectroscopy in living cells: a practical approach. | |
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MedLine Citation:
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PMID: 20013755 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion timescales and the total number of diffusing fluorescent molecules in the cell. A practical step-by-step approach to performing FCS with a commercial spectroscopy/microscopy system, the Zeiss Confocor 3, how to set up live-cell FCS experiments, acquire reliable data, and finally how to analyze the data acquired, are all described in this unit. |
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Authors:
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Nihal Altan-Bonnet; Grégoire Altan-Bonnet |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] Volume: Chapter 4 ISSN: 1934-2616 ISO Abbreviation: Curr Protoc Cell Biol Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-06-04 Revised Date: 2012-05-15 |
Medline Journal Info:
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Nlm Unique ID: 101287856 Medline TA: Curr Protoc Cell Biol Country: United States |
Other Details:
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Languages: eng Pagination: Unit 4.24 Citation Subset: IM |
Copyright Information:
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Copyright 2009 by John Wiley & Sons, Inc. |
Affiliation:
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Department of Biology, Rutgers University, Newark, New Jersey, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Fluorescent Dyes* Humans Research Design Spectrometry, Fluorescence / instrumentation, methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI083408/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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