Document Detail


Fluorescence correlation spectroscopy in living cells: a practical approach.
MedLine Citation:
PMID:  20013755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion timescales and the total number of diffusing fluorescent molecules in the cell. A practical step-by-step approach to performing FCS with a commercial spectroscopy/microscopy system, the Zeiss Confocor 3, how to set up live-cell FCS experiments, acquire reliable data, and finally how to analyze the data acquired, are all described in this unit.
Authors:
Nihal Altan-Bonnet; Grégoire Altan-Bonnet
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]     Volume:  Chapter 4     ISSN:  1934-2616     ISO Abbreviation:  Curr Protoc Cell Biol     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-06-04     Revised Date:  2013-12-23    
Medline Journal Info:
Nlm Unique ID:  101287856     Medline TA:  Curr Protoc Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 4.24     Citation Subset:  IM    
Copyright Information:
Copyright 2009 by John Wiley & Sons, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Fluorescent Dyes*
Humans
Research Design
Spectrometry, Fluorescence / instrumentation,  methods*
Grant Support
ID/Acronym/Agency:
R01 AI083408/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

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