Document Detail


Fluorescence-based transient state monitoring for biomolecular spectroscopy and imaging.
MedLine Citation:
PMID:  20375039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To increase read-out speed, sensitivity or specificity, an often applied strategy in fluorescence-based biomolecular spectroscopy and imaging is to simultaneously record two or more of the fluorescence parameters: intensity, lifetime, polarization or wavelength. This review highlights how additional, to-date largely unexploited, information can be extracted by monitoring long-lived, photo-induced transient states of organic dyes and their dynamics. Two major approaches are presented, where the transient state information is obtained either from fluorescence fluctuation analysis or by recording the time-averaged fluorescence response to a time-modulated excitation. The two approaches combine the detection sensitivity of the fluorescence signal with the environmental sensitivity of the long-lived transient states. For both techniques, proof-of-principle experiments are reviewed, and advantages, limitations and possible applications for biomolecular cellular biology studies are discussed.
Authors:
Jerker Widengren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-04-07
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  7     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-10-20     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  1135-44     Citation Subset:  IM    
Affiliation:
Experimental Biomolecular Physics, Department of Applied Physics, Royal Institute of Technology (KTH), Albanova University Center, Stockholm 106 91, Sweden. jwideng@kth.se
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MeSH Terms
Descriptor/Qualifier:
Diagnostic Imaging / methods*
Fluorescence
Sensitivity and Specificity
Spectrometry, Fluorescence / methods*
Spectrum Analysis / methods
Comments/Corrections

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