| Fluorescence assay for the detection of adherent Candida yeasts to target cells in microtest plates. | |
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MedLine Citation:
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PMID: 8569807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe an assay based on photometric analysis for the measurement of adherence of Candida species to epithelial target cells (Vero cell line). Adherent Candida cells were detected by staining the cells with the fluorescent dye Calcofluor white (CFW), which binds to chitin and glucan in the yeasts. The tests were performed on microtest plates, which were analysed automatically by fluorescence plate readers. The assay is based on the following steps: (i) coating of the microtest plates with target cells (e.g. Vero cells); (ii) infection with Candida: (iii) staining of Candida with CFW; (iv) rinsing to remove non-adherent Candida cells and unbound dye; (v) detection of adherent fluorescent Candida cells. The test was able to detect 4 x 10(4) cells ml-1. The standard deviation was +/- 8%. Day-to-day variation was +/- 10% at most. The adherence of strains of different Candida species was assayed by a standard procedure. The results confirmed the order of adherence, with C. albicans ranking first, followed by C. tropicalis, C. parapsilosis and C. glabrata. |
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Authors:
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M Borg-von Zepelin; T Wagner |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Mycoses Volume: 38 ISSN: 0933-7407 ISO Abbreviation: Mycoses Publication Date: 1995 Sep-Oct |
Date Detail:
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Created Date: 1996-03-06 Completed Date: 1996-03-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8805008 Medline TA: Mycoses Country: GERMANY |
Other Details:
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Languages: eng Pagination: 339-47 Citation Subset: IM |
Affiliation:
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Department of Medical Microbiology, University of Göttingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzenesulfonates / pharmacology Candida / physiology* Candida albicans / physiology* Cell Adhesion* / drug effects Cercopithecus aethiops Dose-Response Relationship, Drug Fluorescent Dyes Microscopy, Fluorescence Species Specificity Vero Cells |
| Chemical | |
Reg. No./Substance:
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0/Benzenesulfonates; 0/Fluorescent Dyes; 4404-43-7/C.I. Fluorescent Brightening Agent 28 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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