Document Detail


Fluorescence assay for the detection of adherent Candida yeasts to target cells in microtest plates.
MedLine Citation:
PMID:  8569807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe an assay based on photometric analysis for the measurement of adherence of Candida species to epithelial target cells (Vero cell line). Adherent Candida cells were detected by staining the cells with the fluorescent dye Calcofluor white (CFW), which binds to chitin and glucan in the yeasts. The tests were performed on microtest plates, which were analysed automatically by fluorescence plate readers. The assay is based on the following steps: (i) coating of the microtest plates with target cells (e.g. Vero cells); (ii) infection with Candida: (iii) staining of Candida with CFW; (iv) rinsing to remove non-adherent Candida cells and unbound dye; (v) detection of adherent fluorescent Candida cells. The test was able to detect 4 x 10(4) cells ml-1. The standard deviation was +/- 8%. Day-to-day variation was +/- 10% at most. The adherence of strains of different Candida species was assayed by a standard procedure. The results confirmed the order of adherence, with C. albicans ranking first, followed by C. tropicalis, C. parapsilosis and C. glabrata.
Authors:
M Borg-von Zepelin; T Wagner
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Mycoses     Volume:  38     ISSN:  0933-7407     ISO Abbreviation:  Mycoses     Publication Date:    1995 Sep-Oct
Date Detail:
Created Date:  1996-03-06     Completed Date:  1996-03-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8805008     Medline TA:  Mycoses     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  339-47     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology, University of Göttingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzenesulfonates / pharmacology
Candida / physiology*
Candida albicans / physiology*
Cell Adhesion* / drug effects
Cercopithecus aethiops
Dose-Response Relationship, Drug
Fluorescent Dyes
Microscopy, Fluorescence
Species Specificity
Vero Cells
Chemical
Reg. No./Substance:
0/Benzenesulfonates; 0/Fluorescent Dyes; 4404-43-7/C.I. Fluorescent Brightening Agent 28

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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