Document Detail


Fluorescein excitation and emission polarization spectra in living cells: changes during the cell cycle.
MedLine Citation:
PMID:  698344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Changes in the fluorescein fluorescence emission and excitation polarization spectra in synchronized cultured S3 fibroblasts at G1, mid-S, and mitosis, as well as in human lymphocytes before and after stimulation with mitogens, were studied. In contrast to those measured in aqueous solutions the emission and excitation polarization spectra in living cells exhibit a wavelength dependence characteristic for the state of the cell cycle. Changes in the temperature and in the amount of intracellular water result in quantitative wavelength-independent changes in the polarization spectra. Possible mechanisms for the qualitative wavelength-dependent changes in the fluorescein emission and excitation polarization spectra during the cell cycle are discussed.
Authors:
L Cercek; B Cercek; C H Ockey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biophysical journal     Volume:  23     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-12-20     Completed Date:  1978-12-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  395-405     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle*
Fibroblasts
Fluoresceins*
Humans
Lymphocytes
Mitogens / pharmacology
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Mitogens
Comments/Corrections

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