Document Detail


Fluidizing the membrane by a local anesthetic: phenylethanol affects membrane protein oligomerization.
MedLine Citation:
PMID:  20970426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The exact mechanism of action of anesthetics is still an open question. While some observations suggest specific anesthetic-protein interactions, nonspecific perturbation of the lipid bilayer has also been suggested. Perturbations of bilayer properties could subsequently affect the structure and function of membrane proteins. Addition of the local anesthetic phenylethanol (PEtOH) to model membranes and intact Escherichia coli cells not only affected membrane fluidity but also severely altered the defined helix-helix interaction within the membrane. This experimental observation suggests that certain anesthetics modulate membrane physical properties and thereby indirectly affect transmembrane (TM) helix-helix interactions, which are not only involved in membrane protein folding and assembly but also important for TM signaling.
Authors:
Veerappan Anbazhagan; Carmen Munz; Lydia Tome; Dirk Schneider
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-21
Journal Detail:
Title:  Journal of molecular biology     Volume:  404     ISSN:  1089-8638     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2010-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  773-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Institut für Pharmazie und Biochemie, Johannes Gutenberg-Universität Mainz, Johann-Joachim-Becher-Weg 30, 55128 Mainz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Anesthetics, Local / metabolism*,  pharmacology
Cell Membrane / drug effects*
Escherichia coli / drug effects*
Escherichia coli Proteins / chemistry,  metabolism
Membrane Fluidity / drug effects*
Membrane Proteins / chemistry,  metabolism*
Models, Biological
Phenylethyl Alcohol / metabolism*,  pharmacology
Protein Binding / drug effects
Protein Interaction Domains and Motifs
Protein Multimerization / drug effects*
Protein Structure, Secondary
Signal Transduction / drug effects
Chemical
Reg. No./Substance:
0/Anesthetics, Local; 0/Escherichia coli Proteins; 0/Membrane Proteins; 60-12-8/Phenylethyl Alcohol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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