| Fludarabine nucleoside induces accumulations of p53, p63 and p73 in the nuclei of human B-lymphoid cell lines, with cytosolic and mitochondrial increases in p53. | |
| | |
MedLine Citation:
|
PMID: 22641291 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
PURPOSE: Human Raji cells treated with fludarabine nucleoside (2-FaraA, 3 μM) undergo apoptosis with accumulation of p53 in the nuclei as multiple phosphorylated isoforms and C-terminal truncated derivatives. Changes induced by 2-FaraA in the levels of p53, p63 and p73 in the nuclear, cytosolic and mitochondrial fractions have been determined in 4 human B-lymphoid cell lines that are TP53-functional (Raji and IM9) and TP53-mutated (MEC1 and U266). EXPERIMENTAL DESIGN: The B-lymphoid cell lines were treated with 2-FaraA (3 μM, 24 h, 48 h) and viability determined. Protein extracts of sub-cellular fractions from 2-FaraA-treated cells were analysed by 1D and 2D electrophoresis, multiple phosphorylated isoforms and truncated derivatives were identified by Western blots for p53, p63 and p73. RESULTS: p53 and p63 were present in all 3 fractions, while p73 was only detected in nuclei. After treatment with 2-FaraA, nuclear p53, p63 and p73 accumulated as multiple phosphorylated isoforms and truncated derivatives. The association of p63 with mitochondria in human cells is novel. CONCLUSIONS AND CLINICAL RELEVANCE: Comprehensive information on the sub-cellular distributions and responses of p53, p63 and p73 to 2-FaraA provides additional insight into mechanisms for induction of apoptosis in the treatment of B-lymphoproliferative disorders with fludarabine. |
| | |
Authors:
|
Juhura G Almazi; Swetlana Mactier; O Giles Best; Ben Crossett; Stephen P Mulligan; Richard I Christopherson |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-5-29 |
Journal Detail:
|
Title: Proteomics. Clinical applications Volume: - ISSN: 1862-8354 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-5-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101298608 Medline TA: Proteomics Clin Appl Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
|
School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Metals on graphene: correlation between adatom adsorption behavior and growth morphology.
Next Document: Comparison of outcomes after allogeneic hematopoietic stem cell transplantation in patients with fol...