| Fluctuations in rat testicular interstitial oxygen tensions are linked to testicular vasomotion: persistence after repair of torsion. | |
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MedLine Citation:
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PMID: 11058542 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Testicular microvascular blood flow is known to exhibit vasomotion, which has been shown to be significantly altered in the short term following the repair of testicular torsion. This loss of vasomotion may ultimately be responsible for the loss of spermatogenesis observed after testicular torsion in rats. In the present study, testicular vasomotion and interstitial oxygen tensions were simultaneously measured prior to, during, and at various time points after repair of testicular torsion in the rat. Testicular torsion was induced by a 720 degrees rotation of the testis for 1 h. Laser-Doppler flowmetry and an oxygen electrode were used to simultaneously measure vasomotion and interstitial oxygen tensions (PO(2)), respectively. Pretorsion control testes had a mean blood flow of 16.3 +/- 1.3 perfusion units (PU) and displayed vasomotion with a cycle frequency of 12 +/- 0.2 cycles per minute and a mean amplitude of 4.2 +/- 0.3 PU. Mean testicular interstitial PO(2) was 12.5 +/- 2.6 mm Hg, which displayed a cyclical variation of 11.9 +/- 0.4 cycles per minute with a mean amplitude of 2.8 +/- 0.8 mm Hg. During the torsion period, both mean blood flow and interstitial PO(2) decreased to approximately zero. Upon detorsion, mean microvascular blood flow and mean interstitial PO(2) values returned to values that were not significantly different from pretorsion values within 30 min; however, vasomotion and PO(2) cycling did not return, even after 24 h. It was 7 days after the repair of torsion before a regular pattern of vasomotion and PO(2) cycling returned. These results demonstrate for the first time a correlation between testicular vasomotion and interstitial PO(2) cycling, and this correlation persists after the repair of testicular torsion. |
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Authors:
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J J Lysiak; Q A Nguyen; T T Turner |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 63 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-15 Completed Date: 2000-12-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1383-9 Citation Subset: IM |
Affiliation:
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Departments of Urology and Cell Biology, University of Virginia Health Science Center, Charlottesville, Virginia 22908, USA. jl6n@virginia.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Capillaries / drug effects, physiology Laser-Doppler Flowmetry Male Muscle, Smooth, Vascular / physiology* Oxygen Consumption / physiology* Rats Rats, Sprague-Dawley Regional Blood Flow / physiology Spermatic Cord Torsion / metabolism*, pathology Testis / blood supply, metabolism*, pathology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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DK-53072/DK/NIDDK NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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