| FluG-dependent asexual development in Aspergillus nidulans occurs via derepression. | |
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MedLine Citation:
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PMID: 16387865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The asexual spore is one of the most crucial factors contributing to the fecundity and fitness of filamentous fungi. Although the developmental activator FluG was shown to be necessary for activation of asexual sporulation (conidiation) and production of the carcinogenic mycotoxin sterigmatocystin (ST) in the model filamentous fungus Aspergillus nidulans, the molecular mechanisms underlying the developmental switch have remained elusive. In this study, we report that the FluG-mediated conidiation in A. nidulans occurs via derepression. Suppressor analyses of fluG led to the identification of the sfgA gene encoding a novel protein with the Gal4-type Zn(II)2Cys6 binuclear cluster DNA-binding motif at the N terminus. Deletion (delta) and 31 other loss-of-function sfgA mutations bypassed the need for fluG in conidiation and production of ST. Moreover, both delta sfgA and delta sfgA delta fluG mutations resulted in identical phenotypes in growth, conidiation, and ST production, indicating that the primary role of FluG is to remove repressive effects imposed by SfgA. In accordance with the proposed regulatory role of SfgA, overexpression of sfgA inhibited conidiation and delayed/reduced expression of conidiation- and ST-specific genes. Genetic analyses demonstrated that SfgA functions downstream of FluG but upstream of transcriptional activators (FlbD, FlbC, FlbB, and BrlA) necessary for normal conidiation. |
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Authors:
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Jeong-Ah Seo; Yajun Guan; Jae-Hyuk Yu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2005-12-30 |
Journal Detail:
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Title: Genetics Volume: 172 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-23 Completed Date: 2009-06-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 1535-44 Citation Subset: IM |
Affiliation:
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Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, Wisconsin 53706, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AAY99779 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Amino Acid Sequence Aspergillus nidulans / genetics, growth & development, physiology* Fungal Proteins / genetics, physiology* Gene Expression Regulation, Fungal / physiology* Molecular Sequence Data Repressor Proteins / genetics, physiology Spores, Fungal / genetics, growth & development, metabolism Suppression, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Repressor Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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