Document Detail


Flowcytometry based characterization of label-retaining stem cell following Transplacental BrdU labeling.
MedLine Citation:
PMID:  20849391     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A method to characterize and culture stem cell from neonate mouse epidermis after transplacental BrdU administration is described. We have characterized stem cell by its properties viz. to retain BrdU-label, adhere rapidly onto collagen-fibronectin substratum, and express a specific biomarker beta-1-integrin. BrdU labeled cells (detected using monoclonal antibody) constituted a sum of 18% of total number of cells. Capability of freshly isolated keratinocytes (LRC) to bind to primary BrdU-antibody or to pick up PI stain was distinguishable. Viable-LRCs did not retain PI. Such cells, termed EpSC, were PI (negative) & BrdU (positive). EpSC constituted 6% of the total cell yield. Culture in low Ca2+ medium and susceptibility to differentiation in presence of high Ca2+ levels further characterized the stem cells. This protocol is useful to study transplacental carcinogenesis.
Authors:
Shiv Poojan; Sushil Kumar
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-9-17
Journal Detail:
Title:  Cell biology international     Volume:  -     ISSN:  1095-8355     ISO Abbreviation:  -     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-9-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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