| Flowcytometry based characterization of label-retaining stem cell following Transplacental BrdU labeling. | |
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MedLine Citation:
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PMID: 20849391 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A method to characterize and culture stem cell from neonate mouse epidermis after transplacental BrdU administration is described. We have characterized stem cell by its properties viz. to retain BrdU-label, adhere rapidly onto collagen-fibronectin substratum, and express a specific biomarker beta-1-integrin. BrdU labeled cells (detected using monoclonal antibody) constituted a sum of 18% of total number of cells. Capability of freshly isolated keratinocytes (LRC) to bind to primary BrdU-antibody or to pick up PI stain was distinguishable. Viable-LRCs did not retain PI. Such cells, termed EpSC, were PI (negative) & BrdU (positive). EpSC constituted 6% of the total cell yield. Culture in low Ca2+ medium and susceptibility to differentiation in presence of high Ca2+ levels further characterized the stem cells. This protocol is useful to study transplacental carcinogenesis. |
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Authors:
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Shiv Poojan; Sushil Kumar |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-9-17 |
Journal Detail:
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Title: Cell biology international Volume: - ISSN: 1095-8355 ISO Abbreviation: - Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-9-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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