Document Detail


Flow-through sampling for electrophoresis-based microfluidic chips using hydrodynamic pumping.
MedLine Citation:
PMID:  11765077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This work presents a novel electrophoretic microchip design which is capable of directly coupling with flow-through analyzers for uninterrupted sampling. In this device, a 3 mm wide sampling channel (SC) was etched on quartz substrate to create the sample inlet and outlet and the 75 microm wide electrophoretic channels were also fabricated on the same substrate. Pressure was used to drive the sample flow through the external tube into the SC and the flow was then split into outlet and electrophoretic channels. A gating voltage was applied to the electrophoretic channel to control the sample loading for subsequent separations and inhibit the sample leakage. The minimum gating voltage required to inhibit the sample leakage depended on the solution buffer and increased with the hydrodynamic flow-rate. A fluorescent dye mixture containing Rhodamine B and Cy3 was introduced into the sample stream at either a continuous or discrete mode via an on-line injection valve and then separated and detected on the microchip using laser-induced fluorescence. For both modes, the relative standard deviation of migration time and peak intensity for consecutive injections was determined to be below 0.6 and 8%, respectively. Because the SC was kept floating, the external sampling equipment requires no electric connection. Therefore, such an electrophoresis-based microchip can be directly coupled with any pressure-driven flow analyzers without hardware modifications. To our best knowledge, this is something currently impossible for reported electrophoretic microchip designs.
Authors:
Y H Lin; G B Lee; C W Li; G R Huang; S H Chen
Related Documents :
7786457 - Blood letting for bsl: the effects of timing and sites on blood volume.
2379117 - Effects of experimenters and different blood sampling procedures on blood metabolite va...
2259827 - Methemoglobinemia associated with loxoscelism.
19455167 - Efficiency and safety of a technique for drawing blood from the hamster cranial vena cava.
9121727 - The effect of circulatory arrest and retrograde cerebral perfusion on microtubule-assoc...
11423057 - Comparison of valve resistance with effective orifice area regarding flow dependence.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  937     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-05-09     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  115-25     Citation Subset:  IM    
Affiliation:
Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Electrophoresis / methods*
Miniaturization

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mechanisms effecting analysis of volatile flavour components by solid-phase microextraction and gas ...
Next Document:  Capillary zone electrophoresis with electrochemical detection--a simple and effective method to anal...