Document Detail


Flow-switching allows independently programmable, extremely stable, high-throughput diffusion-based gradients.
MedLine Citation:
PMID:  23386049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An automated microfluidic cell culture platform that creates and maintains independently programmable diffusion-based gradients is reported. Temporal modulation of the source and sink flow patterns allow generation of extremely stable spatial gradients. We developed a system that integrates 30 parallel gradients in a single device, with 10 different chemical formulations and 3 replicates. Mammalian fibroblast and macrophage cells were screened for NFκB pathway activity under gradients of TNFα, PDGF, and LPS, and multiparameter measurements were performed to demonstrate the capability of the device in dynamic single-cell analysis.
Authors:
Tino Frank; Savaş Tay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lab on a chip     Volume:  13     ISSN:  1473-0189     ISO Abbreviation:  Lab Chip     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-05     Completed Date:  2013-08-29     Revised Date:  2013-12-24    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  1273-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cell Culture Techniques / methods*
Cell Survival / drug effects
Diffusion
Lipopolysaccharides / pharmacology
Mice
Microfluidic Analytical Techniques / methods*
Platelet-Derived Growth Factor / pharmacology
Signal Transduction / drug effects
Transcription Factor RelA / metabolism
Tumor Necrosis Factor-alpha / pharmacology
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 0/Platelet-Derived Growth Factor; 0/Transcription Factor RelA; 0/Tumor Necrosis Factor-alpha
Comments/Corrections
Erratum In:
Lab Chip. 2013 Dec 21;13(24):4891

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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