| Flow sorting of mouse pancreatic B cells by forward and orthogonal light scattering. | |
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MedLine Citation:
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PMID: 6129116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mouse islet cell suspensions were separated into subpopulations based on forward low angle and orthogonal scattered light. Separation fractions were analyzed for hormone content by radioimmunoassays. The pancreatic B-cells had the highest orthogonal light scattering, while they overlapped in forward low angle light scattering with other endocrine (glucagon, pancreatic polypeptide and somatostatin containing cells) and nonendocrine cells. Living and paraformaldehyde fixed islet cell suspensions showed similar distributions of combined light scattering. |
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Authors:
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O Nielsen; J K Larsen; I J Christensen; A Lernmark |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cytometry Volume: 3 ISSN: 0196-4763 ISO Abbreviation: Cytometry Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-02-14 Completed Date: 1983-02-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102328 Medline TA: Cytometry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 177-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Separation / methods* Flow Cytometry Glucagon / analysis Insulin / analysis Islets of Langerhans / cytology* Light Male Mice Pancreatic Polypeptide / analysis Scattering, Radiation Somatostatin / analysis |
| Grant Support | |
ID/Acronym/Agency:
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AM 26190/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 51110-01-1/Somatostatin; 59763-91-6/Pancreatic Polypeptide; 9007-92-5/Glucagon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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