Document Detail


Flow sorting of mouse pancreatic B cells by forward and orthogonal light scattering.
MedLine Citation:
PMID:  6129116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mouse islet cell suspensions were separated into subpopulations based on forward low angle and orthogonal scattered light. Separation fractions were analyzed for hormone content by radioimmunoassays. The pancreatic B-cells had the highest orthogonal light scattering, while they overlapped in forward low angle light scattering with other endocrine (glucagon, pancreatic polypeptide and somatostatin containing cells) and nonendocrine cells. Living and paraformaldehyde fixed islet cell suspensions showed similar distributions of combined light scattering.
Authors:
O Nielsen; J K Larsen; I J Christensen; A Lernmark
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytometry     Volume:  3     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-02-14     Completed Date:  1983-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  177-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Flow Cytometry
Glucagon / analysis
Insulin / analysis
Islets of Langerhans / cytology*
Light
Male
Mice
Pancreatic Polypeptide / analysis
Scattering, Radiation
Somatostatin / analysis
Grant Support
ID/Acronym/Agency:
AM 26190/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 51110-01-1/Somatostatin; 59763-91-6/Pancreatic Polypeptide; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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