Document Detail


Flow sorting of the mouse Cattanach X chromosome, T (X; 7) 1 Ct, in an active or inactive state.
MedLine Citation:
PMID:  7016465     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells containing the mouse T(X;7)1Ct translocation were used to separate active and inactive X chromosomes. Cytophotometric data showed that the X(7) chromosome was at least 15% larger than any normal mouse chromosome. Cloned cell lines were established from male and female embryos with a normal karyotype and carrying the X(7) chromosome in an active or inactive state as shown by replication kinetics. As expected from the cytophotometric data, flow histograms of normal mouse chromosomes stained with 33258 Hoechst had five peaks. A sixth peak was present when the chromosomes came from mice carrying T1Ct. In this peak 6, 30 to 50% of the chromosomes were X(7) and there was almost no contamination by the normal X chromosome.
Authors:
C M Disteche; A V Carrano; L K Ashworth; K Burkhart-Schultz; S A Latt
Related Documents :
15790735 - Localization of am3 using el congenic mouse strains.
7849395 - Ifg, gli, mdm1, mdm2, and mdm3: candidate genes for the mouse pg locus.
22863325 - Chromosome abnormalities in indonesian patients with short stature.
2745715 - Monoclonal antibody based immunoassay for human aminoacylase-1.
2201785 - How toxic is 2,3,7,8-tetrachlorodibenzodioxin to humans?
15920965 - analysis of a paternity case in which the alleged father was deceased: single locus mis...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  29     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1981  
Date Detail:
Created Date:  1981-08-27     Completed Date:  1981-08-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  189-97     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cytological Techniques*
DNA / analysis
DNA Replication
Female
Male
Mice
Sex Chromosomes / physiology*
Spectrometry, Fluorescence
Translocation, Genetic*
X Chromosome / physiology*
Grant Support
ID/Acronym/Agency:
GM 21121/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Olfactory diagnosis.
Next Document:  Indwelling venous catheters. Results of a clinical and experimental study