| Flow-sensitive K+-coupled ATP secretion modulates activity of the epithelial Na+ channel in the distal nephron. | |
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MedLine Citation:
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PMID: 23002235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The epithelial Na(+) channel (ENaC) in the aldosterone-sensitive distal nephron (ASDN) is under tonic inhibition by a local purinergic signaling system responding to changes in dietary sodium intake. Normal BK(Ca) channel function is required for flow-sensitive ATP secretion in the ASDN. We tested here whether ATP secreted through connexin channels in a coupled manner with K(+) efflux through BK(Ca) channels is required for inhibitory purinergic regulation of ENaC in response to increases in sodium intake. Inhibition of connexin channels relieves purinergic inhibition of ENaC. Deletion of the BK-β4 regulatory subunit, which is required for normal BK(Ca) channel function and flow-sensitive ATP secretion in the ASDN, suppresses increases in urinary ATP in response to increases in sodium intake. As a consequence, ENaC activity, particularly in the presence of high sodium intake, is inappropriately elevated in BK-β4 null mice. ENaC in BK-β4 null mice, however, responds normally to exogenous ATP, indicating that increases in activity do not result from end-organ resistance but rather from lowered urinary ATP. Consistent with this, disruption of purinergic regulation increases ENaC activity in wild type but not BK-β4 null mice. Consequently, sodium excretion is impaired in BK-β4 null mice. These results demonstrate that the ATP secreted in the ASDN in a BK(Ca) channel-dependent manner is physiologically available for purinergic inhibition of ENaC in response to changes in sodium homeostasis. Impaired sodium excretion resulting form loss of normal purinergic regulation of ENaC in BK-β4 null mice likely contributes to their elevated blood pressure. |
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Authors:
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Vladislav Bugaj; Steven C Sansom; Donghai Wen; Lori I Hatcher; James D Stockand; Elena Mironova |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-09-21 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 287 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-12 Completed Date: 2013-01-31 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 38552-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Texas Health Sciences Center, San Antonio, Texas 78229, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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chemistry*,
metabolism Animals Blood Pressure Connexins / chemistry Epithelial Sodium Channels / metabolism* Homeostasis Large-Conductance Calcium-Activated Potassium Channel beta Subunits / genetics*, physiology Male Mice Mice, Inbred C57BL Mice, Transgenic Nephrons / metabolism* Patch-Clamp Techniques Sodium / chemistry, metabolism Sodium, Dietary / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK059594/DK/NIDDK NIH HHS; R01 DK71014/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Connexins; 0/Epithelial Sodium Channels; 0/Kcnmb1 protein, mouse; 0/Large-Conductance Calcium-Activated Potassium Channel beta Subunits; 0/Sodium, Dietary; 56-65-5/Adenosine Triphosphate; 7440-23-5/Sodium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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