| Flow photolysis for spatiotemporal stimulation of single cells. | |
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MedLine Citation:
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PMID: 17432827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantitative studies of cellular systems require experimental techniques that can expose single cells to well-controlled chemical stimuli with high spatiotemporal resolution. Here, we combine microfluidic techniques with the photochemical release of caged signaling molecules to generate tailored stimuli on the length scale of individual cells with subsecond switching times. We exemplify this flexible approach by initiating membrane translocation of fluorescent fusion proteins in chemotactic Dictyostelium discoideum cells. |
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Authors:
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Carsten Beta; Danica Wyatt; Wouter-Jan Rappel; Eberhard Bodenschatz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-04-14 |
Journal Detail:
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Title: Analytical chemistry Volume: 79 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-05-14 Completed Date: 2007-07-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 3940-4 Citation Subset: IM |
Affiliation:
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Max Planck Institute for Dynamics and Self-Organization, 37077 Göttingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / metabolism Cells / drug effects* Dictyostelium / cytology Fluorescence Microfluidics / methods* Photolysis* Recombinant Fusion Proteins / pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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