| Flow modulates centriole movements in tubular epithelial cells. | |
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MedLine Citation:
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PMID: 18425533 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Kidney cysts are characterized by an abnormal tubular geometry that may result from loss of orientation and random cell divisions during renal development. Since cystic kidney disease is caused by mutations of ciliary proteins and cilia act as flow sensors in the kidney, we examined three polarized events in Madin Darby Canine Kidney cells under flow: cell division, cell migration, and centriole movement. We found that the mitotic orientation of dividing cells was not affected by flow and was randomly distributed in relation to the direction of the flow. Flow did not alter the direction and speed of cell migration in a wound-healing assay. However, flow resulted in increased motility of centrioles and biased centrioles to move along the axis of the flow. This effect was lost after flow-induced calcium signaling was abolished by a mutant polycystin 2. Our findings suggest that the cilium may translate fluid flow into altered centriole movements to provide tubular epithelial cells with the spatial orientation required to establish and/or maintain a normal tubular geometry. |
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Authors:
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Fruzsina Kotsis; Roland Nitschke; Mara Doerken; Gerd Walz; E Wolfgang Kuehn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-19 |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 456 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-20 Completed Date: 2009-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: Germany |
Other Details:
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Languages: eng Pagination: 1025-35 Citation Subset: IM |
Affiliation:
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Department of Medicine, Renal Unit, University Hospital Freiburg, 79106, Freiburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / physiology Cell Line Cell Movement / physiology Centrioles / physiology*, ultrastructure Centrosome / physiology Cilia / physiology, ultrastructure Cytoplasmic Streaming / physiology* Diffusion Chambers, Culture Dogs Epithelial Cells / physiology*, ultrastructure Fluorescent Dyes Fura-2 Indicators and Reagents Kidney Tubules / cytology, metabolism, physiology* Nephrons / cytology, physiology Plasmids / genetics, physiology Proteins / metabolism Wound Healing / physiology |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Indicators and Reagents; 0/Proteins; 96314-98-6/Fura-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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