Document Detail

Flow-microfluorometric analysis of nuclei isolated from various normal and malignant human epithelial tissues. A preliminary report.
MedLine Citation:
PMID:  894004     Owner:  NLM     Status:  MEDLINE    
In order to obviate some of the technical problems associated with preparation of monocellular cell suspensions required for flow fluorometry, isolation of nuclei from several types of benign and malignant human tissues was undertaken. Satisfactory preparations of nuclei were obtained from epithelia of the uterine cervix and colon and from lung tissue using the citric acid method. The sucrose method was effective with colonic epithelium only. Distribution of deoxyribonucleic acid content in these nuclei was measured based on green fluorescence of acridine orange and red fluorescence of propidium iodide in a Bio-Physics Cytofluorograph. Essentially diploid patterns of deoxyribonucleic acid distribution were observed for all benign samples regardless of tissue origin whereas the malignant samples gave histograms suggestive of abnormal deoxyribonucleic acid distribution. Preliminary observations on distribution of single-stranded nucleic acids using acridine orange red fluorescence showed marked differences between populations of benign and malignant nuclei. Isolated nuclei appear to be suitable for flow-through microfluorometric analysis and offer some significant advantages over intact cells.
L G Koss; R C Wolley; K Schreiber; J Mendecki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  25     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1977 Jul 
Date Detail:
Created Date:  1977-10-28     Completed Date:  1977-10-28     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  565-72     Citation Subset:  IM    
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MeSH Terms
Cell Fractionation
Cell Nucleus / analysis*
Cervix Uteri / cytology
Colon / cytology
Colonic Neoplasms / ultrastructure*
DNA / analysis
DNA, Neoplasm / analysis
Epithelial Cells*
Epithelium / ultrastructure*
Lung Neoplasms / ultrastructure*
Spectrometry, Fluorescence*
Uterine Cervical Neoplasms / ultrastructure*
Reg. No./Substance:
0/DNA, Neoplasm; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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