Document Detail

Flow microfluorometric analysis of alloantigen expression during T cell development.
MedLine Citation:
PMID:  7009163     Owner:  NLM     Status:  MEDLINE    
The fluorescence-activated cell sorter (FACS) was used to examine the expression of several alloantigens on T cells: Thy-1, Lyt-1.1, Lyt-2.1, Ly-5, Ly-6 and Ly-7. Antibodies secreted by monoclonal cell lines were used to characterize the Thy-1:2, Lyt-1.1 and Lyt-2.1 antigenic determinants, whereas Ly-5.1, Ly-6.2 and Ly-7.2 determinants were identified with alloantisera raised by conventional hyperimmunization. Using indirect immunofluorescence with fluorescein isothiocyanate-conjugated reagents, the profiles obtained with the FACS demonstrated that: (a) the expression of Thy-1 is greater on normal thymocytes than on cortisone-resistant thymocytes (CRT), lymph node and spleen cells; (b) in contrast to Thy-1, the expression of Ly-1 on normal thymocytes is less than on CRT, lymph node and spleen cells; (c) about 90% of normal thymocytes abut less than 50% of CRT, spleen and lympho node T cells are Lyt-2+ and (d) although weakly expressed on normal thymocytes, the amount of Ly-6 and Ly-7 present on peripheral T cells was considerably increased. The significance of these findings and the potential for further analysis of T cell developmental pathways is discussed.
T A Potter; P M Hogarth; I F McKenzie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  European journal of immunology     Volume:  10     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1980 Dec 
Date Detail:
Created Date:  1981-05-21     Completed Date:  1981-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  899-903     Citation Subset:  IM    
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MeSH Terms
Cell Count
Cell Differentiation
Cortisone / pharmacology
Immune Sera / pharmacology
Immunologic Techniques*
Isoantigens / classification,  immunology*
Lymph Nodes / cytology
Mice, Inbred BALB C
Mice, Inbred C3H
Spleen / cytology
T-Lymphocytes / classification,  cytology*
Grant Support
Reg. No./Substance:
0/Immune Sera; 0/Isoantigens; 53-06-5/Cortisone

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