Document Detail


Flow microcalorimetry investigation of the influence of glucose and maltose on the growth of Streptococcus agalactiae and the production of CAMP factor (protein B).
MedLine Citation:
PMID:  1865834     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microcalorimetric investigations of the growth of Streptococcus agalactiae and production of CAMP factor were performed in combination with the photometric estimation of bacterial mass and the bioluminescent measurement of extracellular ATP in trypticase peptone-yeast extract broth supplemented with either 2% maltose or glucose. Maltose was found to be a more efficient energy substrate than glucose, providing more energy and C-atoms to the growing cells. In the presence of maltose, the metabolic activity of S. agalactiae was more efficient and better balanced than in that of glucose. Bacterial cells growing in maltose-containing medium used more substrate energy for their anabolic activities, while cells growing in the presence of glucose lost more substrate energy in the form of heat. The addition of 0.2% NaHCO3 to the carbohydrate-supplemented medium enhanced the efficiency of the anabolic activity of growing cells, but did not promote bacterial growth. Microcalorimetry should be considered as a useful alternative as well as a complementary method for the optimization of the growth media or growth conditions.
Authors:
N B Takaisi-Kikuni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbios     Volume:  66     ISSN:  0026-2633     ISO Abbreviation:  Microbios     Publication Date:  1991  
Date Detail:
Created Date:  1991-09-11     Completed Date:  1991-09-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0207257     Medline TA:  Microbios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  173-85     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Robert Koch Institute of the Federal Health Office, Berlin, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis*
Calorimetry
Carbohydrate Metabolism
Culture Media
Glucose / metabolism*
Hemolysin Proteins / biosynthesis*
Hot Temperature
Maltose / metabolism*
Streptococcus agalactiae / growth & development*,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/CAMP protein, Streptococcus; 0/Culture Media; 0/Hemolysin Proteins; 50-99-7/Glucose; 69-79-4/Maltose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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