Document Detail


Flow-injection chemiluminescence determination of epinephrine in pharmaceutical preparations using raw apple juice as enzyme source.
MedLine Citation:
PMID:  18968469     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Raw apple juice exhibits the activity of polyphenol oxidase which can be employed for the determination of mono-, di- and polyhydric phenols. The chemiluminescence detection in the two-channel manifold was used to develop the new procedure for determination of epinephrine. Epinephrine can be determined by this method in pharmaceutical preparations in concentration ranges 1.0-10.0 and 10.0-25.0 mg l(-1), with a detection limit of 0.2 mg l(-1). Good selectivity against typical antioxidants and other coexisting substances was achieved. Relatively slow loss of the enzyme activity was observed during 1 week. The proposed method is very simple. Fresh juice solution from one apple can be prepared in 10 min and used for at least 8 h with excellent precision. Consumption of luminol solution was 0.15 ml min(-1). The throughput was 20 samples per hour.
Authors:
J Michałowski; P Hałaburda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  55     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1165-71     Citation Subset:  -    
Affiliation:
Institute of Chemistry, University of Białystok, al. J. Pilsudskiego 11/4, 15-443 Białystok, Poland.
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