| Flow-injection chemiluminescence determination of epinephrine in pharmaceutical preparations using raw apple juice as enzyme source. | |
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MedLine Citation:
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PMID: 18968469 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Raw apple juice exhibits the activity of polyphenol oxidase which can be employed for the determination of mono-, di- and polyhydric phenols. The chemiluminescence detection in the two-channel manifold was used to develop the new procedure for determination of epinephrine. Epinephrine can be determined by this method in pharmaceutical preparations in concentration ranges 1.0-10.0 and 10.0-25.0 mg l(-1), with a detection limit of 0.2 mg l(-1). Good selectivity against typical antioxidants and other coexisting substances was achieved. Relatively slow loss of the enzyme activity was observed during 1 week. The proposed method is very simple. Fresh juice solution from one apple can be prepared in 10 min and used for at least 8 h with excellent precision. Consumption of luminol solution was 0.15 ml min(-1). The throughput was 20 samples per hour. |
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Authors:
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J Michałowski; P Hałaburda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Talanta Volume: 55 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2008-10-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 1165-71 Citation Subset: - |
Affiliation:
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Institute of Chemistry, University of Białystok, al. J. Pilsudskiego 11/4, 15-443 Białystok, Poland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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