|Flow injection/sequential injection analysis systems: potential use as tools for rapid liver diseases biomarker study.|
|Jump to Full Text|
|PMID: 22518319 Owner: NLM Status: In-Data-Review|
|Flow injection/sequential injection analysis (FIA/SIA) systems are suitable for carrying out automatic wet chemical/biochemical reactions with reduced volume and time consumption. Various parts of the system such as pump, valve, and reactor may be built or adapted from available materials. Therefore the systems can be at lower cost as compared to other instrumentation-based analysis systems. Their applications for determination of biomarkers for liver diseases have been demonstrated in various formats of operation but only a few and limited types of biomarkers have been used as model analytes. This paper summarizes these applications for different types of reactions as a guide for using flow-based systems in more biomarker and/or multibiomarker studies.|
|Supaporn Kradtap Hartwell|
Related Documents :
|15462179 - Vitamin e prevents deleterious effects of di (2-ethyl hexyl) phthalate, a plasticizer u...
23353139 - A new method for flow rate measurement in millimeter-scale pipes.
8708099 - Measurement of liner slips, milking time, and milk yield.
12719609 - Selenium concentration in blood and hair of holstein dairy cows.
3286699 - Relationship of milk proteins in blood with somatic cell counts in milk of dairy cows.
18180119 - Selection of component drug in activating blood flow and removing blood stasis of chine...
1926259 - Effects of nicardipine on cerebral vascular responses to hypocapnia and blood flow velo...
12731949 - Interplay between geometry and flow distribution in an airway tree.
5716849 - Ionic environment of neurones and glial cells in the brain of an amphibian.
|Type: Journal Article Date: 2012-03-18|
|Title: International journal of hepatology Volume: 2012 ISSN: 2090-3456 ISO Abbreviation: Int J Hepatol Publication Date: 2012|
|Created Date: 2012-04-20 Completed Date: - Revised Date: -|
Medline Journal Info:
|Nlm Unique ID: 101564967 Medline TA: Int J Hepatol Country: United States|
|Languages: eng Pagination: 281807 Citation Subset: -|
|Chemistry Department, Xavier University, 3800 Victory Parkway, Cincinnati, OH 45207, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Journal ID (nlm-ta): Int J Hepatol
Journal ID (iso-abbrev): Int J Hepatol
Journal ID (publisher-id): IJHEP
Publisher: Hindawi Publishing Corporation
Copyright © 2012 Supaporn Kradtap Hartwell.
Received Day: 15 Month: 10 Year: 2011
Accepted Day: 17 Month: 12 Year: 2011
Print publication date: Year: 2012
Electronic publication date: Day: 18 Month: 3 Year: 2012
Volume: 2012E-location ID: 281807
PubMed Id: 22518319
|Flow Injection/Sequential Injection Analysis Systems: Potential Use as Tools for Rapid Liver Diseases Biomarker Study|
|Supaporn Kradtap HartwellI1*|
|Chemistry Department, Xavier University, 3800 Victory Parkway, Cincinnati, OH 45207, USA
|Correspondence: *Supaporn Kradtap Hartwell: email@example.com
[other] Academic Editor: Neil Guha
Liver diseases (hepatic diseases) cover the broad range of liver disorders. The liver has many important functions including breaking down toxic substances in the body and excreting them into urine, producing and secreting bile to aid in food digestion, converting glucose into stored glycogen and vice versa, metabolizing ingested medicines to obtain active ingredients, and producing blood-clotting factors, amino acids and cholesterol to transport fat. Damage to the liver will cause disruption of these functions which can cause serious damage to the body. Even though the liver is considered the only organ in the body that has exceptional capability in replacing damaged cells, if too many liver cells are damaged, the liver may fail to perform properly. Symptoms caused from liver failure may not be obvious until many liver cells (up to 75%) have malfunctioned . Detection of liver problems at an early stage would increase the chances of curing them. Liver biopsy is currently the best method for diagnosis of chronic liver diseases [2, 3]. However, biopsy procedure involves the rather invasive method of taking a small piece of liver tissue to be examined under a microscope. The repetition of liver biopsy during diagnosis and treatment is hard on patients. Since liver diseases can also affect almost all other body systems, many substances/chemicals in the body may respond to the malfunctioning of the liver. The use of these substances/chemicals as biomarkers has become an area of high interest in research as scientists look for alternative noninvasive diagnostic approaches [2, 4, 5]. Even though none of the biomarkers alone nor the liver biopsy is perfect, they can be used for indication whether further investigation is required .
Studies for the effectiveness of biomarkers involve the collection of numerous samples which may be available at different periods of time. Then the analyses of those samples are usually carried out using the same method for comparison. Most of the possible biomarkers being proposed and under study are biomolecules such as proteins and enzymes [6–9]. Analysis of biomarkers in samples with complicated matrices like body fluids requires highly specific and sensitive techniques. Immunoassay is one of the most widely used techniques for these purposes . It can be used to quantify proteins, enzymes, and other biomolecules owing to the flexibility of the immunoassay format and the available antibodies. Fluorescence- and chemiluminescence-based techniques are also of interest due to their high sensitivity [11, 12]. However, these techniques are time consuming and require a skillful operator. From the many possible biomarkers proposed for liver diseases, only a few are accepted for use and only one which is called fucosylated Alpha-fetoprotein (AFP) is a United States Food and Drug Administration approved biomarker [13, 14]. The study of biomarkers for any disease seems to have the same common problem of unreliable results. This is mainly due to having insufficient numbers of sample subjects and insufficient diverse population as well as the lack of reliable and easy methods to manage various sets of samples and to validate analytical methods [13, 15]. On the other hand, since each research group around the world has access to different small sets of samples, it may be useful for the various groups to conduct their studies using different methods. Even though the results cannot be compared directly, the accumulated studies of those results/conclusions about the same biomarker obtained from different analysis methods should help the medical community in drawing conclusions about the trends and effectiveness of a particular biomarker. The effectiveness of the biomarkers should be revealed with similar significance, no matter which methods were used to quantify them.
Analysis technology has evolved into more rapid and low volume operations. The automatic features of the analysis system that can handle tedious and time-consuming analysis protocols are in demand. This paper is focused on the flow-based analysis techniques called flow injection/sequential injection analysis (FIA/SIA) for use as alternative systems for automatic and rapid quantification of biomarkers for liver diseases. The relatively low cost of these semi to fully automatic systems may help broaden opportunities for medical care/clinical studies in remote areas where medical personnel are limited and also provide additional information/results about the effectiveness of the candidate biomarkers.
Flow injection analysis technique has been employed to automate a wide variety of chemical/biochemical analyses since its invention in the early 1970s . Owing to its feasibility in coupling with various types of detectors, the applications are numerous. Since parts of the systems can be made in house or replaced as suitable, the cost of the systems is relatively lower than many other commercial instrumentation-based analysis systems. This makes flow injection/sequential injection analysis systems especially suitable for low-budget laboratories. A simple flow injection system, see Figure 1, is composed of a pump for drawing solutions (reagents) into the system via pump tubing. An injection valve is used as an introduction port for sample to merge into the stream of continuously flowing reagent. Detectable product is formed while flowing and simultaneously it enters the detector and then proceeds out to waste. Various types of pump and valve may be used. The most common types are peristaltic pump and a six-port switching valve (similar to one used in HPLC system). Unlike most commercial instrumentation-based analysis systems, the FIA and SIA systems may be built/assembled in house. The beneficial feature of flow injection systems is the flexibility of the formats and designs that can be created based on applications. Various companies offer parts (i.e., pumps, valve, detector, tubing, nuts, and ferrule) for various purposes. These parts can be adapted for use in flow-based systems depending on budget and the flow system design. For example, in the early years of FI development and application in low-budget laboratories, an IV bag hung at a certain height and a cheap aquarium pump were reported as successfully used in place of a peristaltic pump . Lower-cost solenoid valves or plastic 3-way valves and tubing may also be used to construct a hydrodynamic injection system instead of using a more expensive commercial 6-port valve .
Reagent may be used as a carrier, where the sample is injected into the reagent stream. However, if the reagent is high cost and the sample is abundant, reverse FIA  may be carried out by using the sample solution as the carrier stream and introducing reagent at the injection point. The product is formed while reagent and sample flow together in the small tubing after merging at the sample injection point. The product zone, see Figure 2, has concentrated product in the middle of the zone, and it is more diluted on both sides of the zone due to dispersion of the solution plug in the carrier stream. Therefore, when the product zone flows through the detector, the beginning of the zone (part A) with low product concentration will enter first, followed by the high product concentration middle zone (part B) and the end of zone with low product concentration (part C). The resultant signal shows as peak signal, called FIA gram, where the highest point resulted from the highest product concentration in the middle of the zone. Detection is done much earlier before steady state; therefore the analysis time is dramatically reduced as compared to conventional batch-wise analyses where detection is normally done after the reaction is completed or has reached steady state. The constant flowrate enables the detection of repetitive analyses to be done at the same point of time. Thus, even though not at steady state, the resultant FIA gram (peak height or peak area) can be precisely related to the quantity of sample.
Later generations of flow injection analysis technique incorporate many pumps, valves, and tubing to accommodate more complicated chemical reactions that need many reagents. The latest generation, called sequential injection analysis , see Figure 3, has a downscaled system that consumes even smaller volumes of reagents and samples in a few μL level with the use of a bidirectional syringe pump and multiports selection valve. Reagents and sample can be drawn sequentially and stacked into the mixing coil before mixing while being pushed in reverse direction into the detector. The operational steps from sample introduction, chemical reaction, to detection are fully automated and precisely controlled with computer software. The system can be programmed to stop for a desired period of time; therefore, the study of slow reactions and those that require incubation time such as immunoassay is possible. Accessories such as lab-on-valve (LOV) unit with ports for attaching a fiber optic spectrophotometric detector introduce more areas of applications with real time detection [21–23].
Most research groups have reported that the flow-based systems not only increase sample throughput but also reduce the consumption of sample and reagents. This may be a suitable approach for cases where body fluid/blood samples are limited or need to be divided for various other tests. As compared to most conventional bench top wet chemistry, flow injection requires a lot less sample volume. For example, in titration, sample volume in batch method is in mL whereas in flow-based titration, sample volume injected is in μL . A direct comparison between volumes used can only be made when considering the same analyte and detection methods. Some downscaled batch methods are able to reduce the volume to μL, but in general, FI usually requires relatively less sample volume for a particular analyte or sample being studied. For example, the osmotic fragility test (OFT) of red blood cells normally requires 20 μL of undiluted blood sample in batch spectrometric method whereas only 1 μL of undiluted sample is required in the FI system where it is tested in 100-fold dilution . As compared to standard bioassay technique such as ELISA, the volume required by flow-based systems is also usually lower. For example, the assay of hyaluronan in serum using SI required 10 μL of serum sample, as compared to the conventional routine microplate assay that requires 120 μL of serum sample . For some commercially available ELISA kits such as the cytokeratin 18 (CK18) biomarker kit which requires 50 μL of sample , no direct comparison of sample volume usage can be made unless those samples have been tested within the flow-based system, but lower sample volume would be expected in the flow-based system. Most lateral flow chromatography kits may require only a drop of sample, but they normally only yield a simple yes/no answer without any detail of quantity.
Table 1 summarizes the works that employed flow injection/sequential injection systems and microfluidics devices for rapid quantitative analysis of some substances that have been reported as candidate biomarkers for liver diseases. Most works emphasize the improved sample throughput. Most works also demonstrated very high precision, reported as percent relative standard deviation (%RSD), as shown in Table 1. Various possible ways of operation and detection using flow-based systems are described.
A flow injection analysis system can be used simply as an automatic system to carry out the mixing of sample and reagent(s). The product formed simultaneously flows into the detector. Depending on the reaction involved, product can be detected by coupling the flow injection system with suitable detectors (e.g., fluorescence spectrometer [28–30], UV-Vis spectrometer [31, 32], Rayleigh light scattering [33–35], or amperometer ). Normally, the detection cells for the detectors are modified to be compatible with the continuous flow of solution in the flow systems by having inlet and outlet tubings, and they are commercially available with various volumes and formats [37, 38]. Fluorescence is highly sensitive; therefore, it has been employed as a detector for the analysis systems that involve very low product volume such as in a nanofluidic system . Sakai et al. demonstrated the formation of micelle in the flow line using nonionic surfactant Triton X-100 (amphipathic molecules that arrange themselves in spherical form in aqueous solution with hydrophilic ends outwards and hydrophobic ends inward)  and also the successive determination of multianalytes (e.g., human serum albumin and glucose) from the same sample . Even though the samples used in the report were from diabetic subjects, it is clear that the same key idea of multianalytes detection can be adapted for liver diseases cases as well.
A microcolumn packed with specific reagent-coated microbeads can be used as a reactor to accommodate the chemical/biochemical reaction. For example, Gao et al.  utilized packed columns with enzyme-coated beads to carry out multisteps enzymatic reactions. Another reported type of reactor  is a commercial hollow fiber reactor with cuprammonium rayon membrane for immobilization of enzyme. These works sought detection of bile acids in urine and serum, respectively. Flow injection facilitated the introduction of sample solution and reagent into the reactor and simultaneously transported the colored/luminescent product to the detector.
Many biomarkers are protein or enzyme which normally can be determined using immunoassay technique. Conventional immunoassay technique is carried out in microplate format where multisteps incubations and washing are done in an array of small plastic wells, each accommodating 100–500 μL volume of solution. The test requires skillful lab personnel to obtain precise and accurate results from handling the microvolume solution and ensuring precise incubation/washing time and volume for each well. The immunoassay process usually takes 3–8 hours depending on detailed steps. The good point of microplate immunoassay format is that many samples can be analyzed in parallel. However, in many circumstances, a small number of samples may need to be analyzed with the demand of quick results. In addition, in many areas of the world, skilled medical personnel are not available to conduct such a complicated test. More automated immunoassay systems where the volume and time are controlled by using a constant flow rate pump to introduce and to draw solutions from the reaction cell have been reported in various formats .
To change the format from conventional static immunoassay system to dynamic flow formats, the reaction cell has to be changed from being the wall of a microwell plate to other forms of solid surfaces that can be accommodated easily in the flow of solution. Microbeads and capillary are the main types reported. An example for the application in biomarkers research is sequential injection-glass capillary immunoassay [26, 43]. The sequential injection system was used to precisely control the incubation time and small volume of solution in the range of 10–80 μL which is even smaller than some of those used in conventional microwell plate format. A glass capillary was easily connected to the system as part of the tubing that the solution conveniently flows through without any back pressure which may occur when using beads. The wall of the glass capillary was used as the solid surface for immobilization of biomolecules to be employed in subsequent steps of competitive immunoassay.
Microbeads are used in immunoassay with the capability to increase surface area to improve sensitivity. However, beads that are packed or trapped in the reactor can cause back pressure inside the flow system, so a slow flow rate should be used. Example of sequential injection bead-based immunoassay system was reported for determination of hyaluronan, a possible biomarker for liver diseases . The lab-on-valve (LOV) unit with on-valve fiber optic spectrometer can also be used in conjunction with functionalized beads that are trapped in the LOV unit to obtain direct, real time detection . Although, there is no work reported on its application for study of liver disease biomarkers, the possibility exists for employing LOV bead immunoassay for such a task. Nevertheless, employing beads in the flow system would require more sophisticated control systems than most low-budget laboratories could devise unless pre-existing equipment were to be adapted.
The use of microbeads in another aspect, other than using them as a solid surface for immobilization of biomolecules as used in immunoassay, is reported as a preconcentration surface to accumulate analyte of interest before detection. The flow of solution within an easily designed cell for trapping beads and releasing beads when needed is called a flow injection-bead injection system. An example of such a system for alkaline phosphatase in human serum was demonstrated . Even though the work used beads coated with wheat germ lectin for specific binding for bone-alkaline phosphatase, it should be able to combine with total alkaline phosphatase test to estimate for liver alkaline phosphatase. The use of membrane for the same purpose of improvement of sensitivity was also reported. With-state-of-the art development in nanotechnology, nano-pore membrane used with electrokinetic fluid flow as a nanofluidic protein accumulator was claimed to offer a much higher sensitivity in the analysis of human serum albumin than other methods .
By using a detection method that can measure the differences of surface properties before and after binding to the target analyte, such as surface plasmon resonance (SPR) technique, the amount of bound analyte on the surface could be quantitated directly without the need to add any reagents. Aoki and Toyama  demonstrated this system by determination of human serum albumin in urine using gold as an adsorption surface. A flow injection system was employed for continuously feeding the sample containing uric protein onto the gold surface.
As can be observed from Table 1, most published works that demonstrated flow-based systems for liver diseases biomarkers used the same limited types of target analytes, that is, serum albumin and total protein. There are many substances in body fluids that have been reported as potential biomarkers for liver diseases. Determination of these different biomarkers at the same time may provide better conclusion about the existence of the diseases. Therefore, future studies using flow injection/sequential injection systems should focus on various other possible biomarkers as well as applications for conducting simultaneous detection of multibiomarkers from one shot of sample. Trends in development of analytical devices have also been gearing toward a point of care purpose. The main challenges are to develop the system for solution introduction with controllable flow rates, effective reagent mixing, and detection unit in downscaled format that can be integrated into compact stand-alone devices.
Various formats of flow injection/sequential injection analysis and micro-/nanofluidic systems can be set up to study biomarkers. Even though few works have reported on the study of liver disease biomarkers using flow-based systems, works related to determination of protein and enzymes are numerous and should be adaptable for studies of liver disease biomarkers. These flow-based systems are versatile and can be used as an alternative method for rapid screening of biomarkers to aid in disease diagnosis. Their low-volume consumption is particularly suitable for the study of a biomarker, in which samples may be divided for many other tests, either to evaluate different biomarkers or to accompany the initial biomarker test.
The facility and financial support from the Chemistry Department, Xavier University is acknowledged.
|1.||Wedro B. Liver disease (Hepatic disease)Year: 2011 http://www.medicinenet.com/liver_disease/article.htm.|
|2.||Poynard T,Morra R,Ingiliz P,et al. Biomarkers of liver fibrosisAdvances in Clinical ChemistryYear: 20084613116019004189|
|3.||Gangadharan B,Antrobus R,Dwek RA,Zitzmann N. Novel serum biomarker candidates for liver fibrosis in hepatitis C patientsClinical ChemistryYear: 200753101792179917702858|
|4.||Abdel-Ghaffar TY,Behairy BE,Abd El-Shaheed A,et al. Clinical benefits of biochemical markers of fibrosis in Egyptian children with chronic liver diseasesGastroenterology ResearchYear: 20103262271|
|5.||Maor Y,Bashari D,Kenet G,et al. Non-invasive biomarkers of liver fibrosis in haemophilia patients with hepatitis C: can you avoid liver biopsy?HaemophiliaYear: 200612437237916834736|
|6.||Stolzke T. Liver biomarkerYear: 2011 http://www.liver-products.com/liver-diagnostic/liver-biomarker.html.|
|7.||Stibbe KJM,Verveer C,Francke J,et al. Comparison of non-invasive assessment to diagnose liver fibrosis in chronic hepatitis B and C patientsScandinavian Journal of GastroenterologyYear: 2011467-896297221623677|
|8.||de Ávila RE,Carmo RA,de Farah KP,et al. Hyaluronic acid in the evaluation of liver fibrosis in patients with hepatitis C on haemodialysisBrazilian Journal of Infectious DiseasesYear: 201014433534120963316|
|9.||Faith M,Eapen CE,Wilfred G,Ramachandran J,Jacob M. Serum biotinidase is a sensitive and specific biochemical marker of hepatic dysfunction: a preliminary reportHepatology ResearchYear: 2007371131717300694|
|10.||Diamandis EP,Christopoulos TK. ImmunoassayYear: 1996London, UKAcademic Press|
|11.||Soetebeer UB,Schierenberg MO,Möller JG,et al. Capillary electrophoresis with laser-induced fluorescence in clinical drug development—routine application and future aspectsJournal of Chromatography AYear: 20008951-214715511105856|
|12.||Wong DKH,Tanaka Y,Lai CL,Mizokami M,Fung J,Yuen MF. Hepatitis B virus core-related antigens as markers for monitoring chronic hepatitis B infectionJournal of Clinical MicrobiologyYear: 200745123942394717942661|
|13.||Benowitz S. Liver cancer biomarkers struggling to succeedJournal of the National Cancer InstituteYear: 200799859059117440159|
|14.||Choi J,Park Y,Kim J-H,Kim H-S. Evaluation of revisited fucosylated alpha-fetoprotein (AFP-L3) with an autoanalyzer μTAS in a clinical laboratoryClinica Chimica ActaYear: 20124131-2170174|
|15.||Chau CH,Rixe O,McLeod H,Figg WD. Validation of analytic methods for biomarkers used in drug developmentClinical Cancer ResearchYear: 200814195967597618829475|
|16.||Ruzicka J,Hansen EH. Flow injection analysis: from beaker to microfluidicsAnalytical ChemistryYear: 2000725|
|17.||Grudpan K,Taylor C,Sitter H,Keller C. Flow injection analysis using an aquarium air pumpFresenius’ Journal of Analytical ChemistryYear: 199334610-11882884|
|18.||Kradtap Hartwell S,Kochasit W,Kerdphon S,et al. Hydrodynamic sequential injection system for a rapid dichlorophenol indophenol precipitation test for hemoglobin EMicrochimica ActaYear: 20091673-4201209|
|19.||GrÜnhut M,Palomeque ME,Lista AG,Band BSF. Enzymatic reverse FIA method for total phenols determination in urine samplesTalantaYear: 20077141520152319071485|
|20.||FIA Global. FIA/SIA tutorial lesson 7: sequential injection analysisYear: 2009 http://www.globalfia.com/index.php?option=com_content&view=article&id=54&Itemid=86.|
|21.||Luque de Castro MD,Ruiz-Jiménez J,Pérez-Serradilla JA. Lab-on-valve: a useful tool in biochemical analysisTrends in Analytical ChemistryYear: 2008272118126|
|22.||Vidigal SSMP,Tóth IV,Rangel AOSS. Sequential injection lab-on-valve system for the determination of the activity of peroxidase in vegetablesJournal of Agricultural and Food ChemistryYear: 20105842071207520108899|
|23.||Lee PL,Sun YC,Ling YC. Magnetic nano-adsorbent integrated with lab-on-valve system for trace analysis of multiple heavy metalsJournal of Analytical Atomic SpectrometryYear: 2009243320327|
|24.||Jakmunee J,Pathimapornlert L,Kradtap Hartwell S,Grudpan K. Novel approach for mono-segmented flow micro-titration with sequential injection using a lab-on-valve system: a model study for the assay of acidity in fruit juicesAnalystYear: 2005130329930315724157|
|25.||Khonyoung S,Kradtap Hartwell S,Jakmunee J,Lapanantnoppakhun S,Sanguansermsri T,Grudpan K. A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: possibility for automatic screening of beta-thalassemia traitAnalytical SciencesYear: 200925681982419531894|
|26.||Kradtap Hartwell S,Boonmalai A,Kongtawelert P,Grudpan K. Sequential injection-immunoassay system with a plain glass capillary reactor for the assay of hyaluronanAnalytical SciencesYear: 2010261697420065590|
|27.||Cytokeratin CK-18 ELISA kit (catalog No. ABIN366976) protocolYear: 2011 http://www.antibodies-online.com/kit/366976/cytokeratin+18+CK-18+ELISA/#references.|
|28.||Huang CB,Zhang K,Liu XL,Wang SF. A flow-injection chemiluminescence method for the determination of human serum albumin, using the reaction of fluorescein-human serum albumin-sodium hypochlorite by the enhancement effect of the cationic surfactant cetyltrimethylammonium bromideLuminescenceYear: 200722539340017471452|
|29.||Chen X,Wang J. A sequential injection fluorometric procedure for rapid determination of total protein in human serumTalantaYear: 200669368168518970622|
|30.||Wu D,Steckl AJ. High speed nanofluidic protein accumulatorLab on a ChipYear: 20099131890189619532964|
|31.||Sakai T,Kito Y,Teshima N,Katoh S,Watla-Iad K,Grudpan K. Spectrophotometric flow injection analysis of protein in urine using tetrabromophenolphthalein ethyl ester and triton X-100Journal of Flow Injection AnalysisYear: 2007242326|
|32.||Kradtap Hartwell S,Somprayoon D,Kongtawelert P,et al. Online assay of bone specific alkaline phosphatase with a flow injection-bead injection systemAnalytica Chimica ActaYear: 20076001-218819317903483|
|33.||Li Y,Dong L,Zhang Y,Hu Z,Chen X. Determination of proteins by flow injection analysis coupled with the Rayleigh light scattering techniqueTalantaYear: 200771110911419071275|
|34.||Dong L,Li Y,Zhang Y,Chen X,Hu Z. Determination of proteins in human serum by combination of flow injection sampling with resonance light scattering techniqueMicrochimica ActaYear: 20071591-24955|
|35.||Li Y,Dong L,Wang W,Hu Z,Chen X. Flow injection analysis-Rayleigh light scattering detection for online determination of protein in human serum sampleAnalytical BiochemistryYear: 20063541646916712768|
|36.||Morimoto K,Upadhyay S,Higashiyama T,et al. Electrochemical microsystem with porous matrix packed-beds for enzyme analysisSensors and Actuators BYear: 20071242477485|
|37.||Bioanalytical System (BASi)BASi LCEC flowcellsYear: 2011 http://www.basinc.com/products/ec/flowcells.php.|
|38.||Sigma-Aldrich ProductsHellma flow-through cellYear: 2011 http://www.sigmaaldrich.com/labware/labware-products.html?TablePage=17213790.|
|39.||Watla-iad K,Sakai T,Teshima N,Katoh S,Grudpan K. Successive determination of urinary protein and glucose using spectrophotometric sequential injection methodAnalytica Chimica ActaYear: 2007604213914617996535|
|40.||Gao XF,Li YS,Karube I. Flow injection spectrophotometric determination of sulfated bile acids in urine with immobilized enzyme reactors using water soluble tetrazolium blue-5Analytica Chimica ActaYear: 20014432257264|
|41.||Oda K,Yoshida S,Hirose S,Takeda T. Determination of total 3α-hydroxy bile acids in serum by a bioluminescent flow-injection system using a hollow-fibre reactorAnalytica Chimica ActaYear: 19892252273282|
|42.||Kradtap Hartwell S,Grudpan K. Flow based immuno/bioassay and trends in micro-immuno/biosensorsMicrochimica ActaYear: 20101693201220|
|43.||Khonyoung S,Reanpang P,Kongtawelert P,et al. Sequential injection system with modified glass capillary for automation in immunoassay of chondroitin sulfateAnalytical LettersYear: 2011441–3327339|
|44.||Kradtap Hartwell S,Srisawang B,Kongtawelert P,Jakmunee J,Grudpan K. Sequential injection-ELISA based system for online determination of hyaluronanTalantaYear: 200566252152718970016|
|45.||Wang J,Hansen EH. Sequential injection lab-on-valve: the third generation of flow injection analysisTrends in Analytical ChemistryYear: 2003224225231|
|46.||Aoki K,Toyama S. Approach of simple uric protein sensor by the use of direct protein adsorption onto gold surface (abstract)Chemical SensorsYear: 200420142144|
Previous Document: Treatment of liver metastases in patients with neuroendocrine tumors of gastroesophageal and pancrea...
Next Document: Potential Antioxidant Role of Tridham in Managing Oxidative Stress against Aflatoxin-B(1)-Induced Ex...