| Flow injection analysis of blood L-lactate by using a Prussian Blue-based biosensor as amperometric detector. | |
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MedLine Citation:
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PMID: 17449005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An amperometric l-lactate biosensor was fabricated by confining lactate oxidase in a Prussian Blue-modified electrode with a Nafion membrane. The detector was assembled in a flow injection apparatus and operated at -0.1 V. Conditions for optimal electrode response were determined by investigating the influence of the amount of immobilized enzyme, the sample volume, and the flow rate. At the established operational conditions, the biosensor exhibited negligible response from interfering species usually present in biological fluids. The stability of the biosensor was also investigated, and its sensitivity was maintained unchanged at certain experimental conditions. l-Lactate was determined in blood samples, and the influence of physical exercise on the results was clearly evidenced, demonstrating that the proposed amperometric detector is suitable for monitoring changes in the l-lactate levels in biological fluids. |
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Authors:
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Denise Lowinsohn; Mauro Bertotti |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-18 |
Journal Detail:
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Title: Analytical biochemistry Volume: 365 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-05-14 Completed Date: 2007-08-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 260-5 Citation Subset: IM |
Affiliation:
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Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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methods* Electrochemistry / methods* Ferrocyanides / analysis* Flow Injection Analysis / methods* Humans Lactic Acid / blood* Reproducibility of Results Sensitivity and Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Ferrocyanides; 14038-43-8/ferric ferrocyanide; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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