| Flow-induced shear stresses increase the number of cell-cell contacts within extracellular matrix. | |
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MedLine Citation:
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PMID: 18470918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The formation of cell-cell contacts within extracellular matrix (ECM) is essential to maintain tissue homeostasis and metabolism, as well as critical toward the cell-ECM mechanotransduction that can affect intracellular organization and intercellular communication to enable cell response to external stimuli. This work illustrates the effects of shear stresses on cell-cell contacts within pre-stressed collagen ECM that were loaded in two separate conditions of constant flow (CF) and constant elution time (CET). The numbers of cell-cell contacts and cytoplasmic processes in both media and 3D ECM gels were analyzed in order to examine the shear effects of different magnitudes and time periods on 3D cell-ECM formation. The sheared collagen ECM microstructures were imaged and studied via scanning electron microscopy (SEM) to illustrate greater distances between constituent cells when larger shear stresses were applied. And the gap junction Connexin 43 expressed between networked cells that were sheared in short time period using CF loading exhibited more than those using CET loading. Notably, the number of cell-cell contacts increased when larger shear stresses were applied, suggesting these stresses may be used to increase intercellular communication within 3D matrixes. |
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Authors:
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Qingjun Kong; Maribel Vazquez |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 89 ISSN: 1552-4965 ISO Abbreviation: - Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-11 Completed Date: 2009-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 968-79 Citation Subset: IM |
Copyright Information:
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Copyright 2008 Wiley Periodicals, Inc. |
Affiliation:
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Department of Mechanical Engineering, The City College of The City University of New York (CCNY), 140th Street and Convent Ave., T-402, New York, New York 10031, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Communication* Cell Surface Extensions / metabolism Collagen / ultrastructure Elasticity Extracellular Matrix / metabolism* Fibroblasts / cytology* Rheology Solutions Stress, Mechanical* Time Factors Viscosity |
| Grant Support | |
ID/Acronym/Agency:
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GM071702-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Solutions; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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