Document Detail

Flow inconsistency: the evil twin of column switching--hardware aspects.
MedLine Citation:
PMID:  19765715     Owner:  NLM     Status:  MEDLINE    
Solvent flow, generated by HPLC pumps is consistent and accurate. This statement, while true for single column (one dimensional) liquid chromatography applications, may not apply to column switching applications. Connection of pumps and/or columns to one flow path may cause substantial pressure changes. Immediate post valve switch pressure differences between pumps can cause backflow where the mobile phase stored at higher pressure will temporary flow into the lower pressure area. A more common side effect of column switching is flow inconsistency during pump pressurization. For the duration of pump pressurization, liquid flow through the column will be smaller than expected since the HPLC column acts like a flow restrictor.
Eduard Rogatsky; Kara Braaten; Greg Cruikshank; Harsha Jayatillake; BingNa Zheng; Daniel T Stein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-09-01
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1216     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-19     Completed Date:  2009-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  7721-7     Citation Subset:  IM    
Analytical Core Lab, Institute for Clinical and Translational Research, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Ave., Bronx, NY 10461, USA.
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MeSH Terms
Chromatography, High Pressure Liquid / instrumentation*
Equipment Design
Grant Support

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