Document Detail


Flow and image cytometry for quality assessment of fresh and frozen human sperm samples.
MedLine Citation:
PMID:  2275877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A previous study reported that sperm mitochondrial activity and sperm motility can be evaluated by combined flow and image cytometry, suggesting their potential interest in fertility clinical applications and for studying the effect of physical and chemical agents that modify sperm motility and/or metabolism. This paper focuses on the effect of freezing sperm in liquid nitrogen, extensively used in artificial insemination (AI), combined with different sperm manipulations (washing, swim-up in capacitating medium, CM) using flow and image cytometry on sperm samples from three fertile donors. Rhodamine 123 (Rh123) uptake profiles were bimodal both for fresh and frozen/thawed samples. The mean value of fluorescence of active cells (m+) remained nearly similar after freezing while the percentage of active cells (%C+) was significantly decreased and the percentage of dead cells (%dc, revealed by propidium iodide uptake) significantly increased. In all experiments, the decrease of MOT (percentage of motile sperm) due to freezing was concomitant, to a smaller extent, with a drop in %C+ and an increase in %dc; there was a good relationship between velocity (VCL, VSL) or trajectory characteristics (ALH) and the mean fluorescence values of active cells (m+). Sperm immobilization was neither found related to a major decrease of Rh123 fluorescence nor to an increase in dead cells.
Authors:
X Ronot; J Auger
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology     Volume:  2     ISSN:  0921-8912     ISO Abbreviation:  Anal Cell Pathol     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1991-03-05     Completed Date:  1991-03-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911016     Medline TA:  Anal Cell Pathol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
Affiliation:
Laboratoire de Pharmacologie Cellulaire, Centre de Recherches Biomédicales des Cordeliers, Ecole Pratique des Hautes Etudes, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Cryopreservation*
Flow Cytometry
Fluorescent Dyes / diagnostic use
Humans
Image Processing, Computer-Assisted
Male
Mitochondria / metabolism
Rhodamine 123
Rhodamines / diagnostic use
Sperm Motility
Spermatozoa / metabolism,  physiology*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Rhodamines; 62669-70-9/Rhodamine 123

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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