Document Detail

Flow and image cytometric DNA analysis in Ewing's sarcoma.
MedLine Citation:
PMID:  1542636     Owner:  NLM     Status:  MEDLINE    
Ewing's sarcoma is the second most common bone tumor in childhood, with an overall 5-yr survival of 40%. It is one of the poorly differentiated small spherical cell tumors frequently requiring distinction from rhabdomyosarcoma, neuroblastoma, osteosarcoma, primitive neuroectodermal tumor, and lymphoma. The majority of rhabdomyosarcomas, neuroblastomas, and osteosarcomas are aneuploid, whereas Ewing's sarcomas are usually diploid. To determine whether there is any correlation between DNA content, morphology, site, and survival in Ewing's sarcoma and extraosseous Ewing's sarcoma, 21 tumor samples were studied retrospectively (3 extraosseous Ewing's and 18 Ewing's sarcomas). The DNA analysis was performed on disaggregated paraffin-embedded tissue nuclei by flow (FCM) and image (IC) cytometry and correlated with the histology and clinical history. The DNA ploidy by FCM on 17 of 18 Ewing's sarcoma samples was 12 diploid, 1 aneuploid, and 4 tetraploid. By IC, the DNA ploidy on 16 samples was 13 diploid, 1 aneuploid, and 2 tetraploid. Three samples were nonevaluable (1 by FCM and 2 by IC). The agreement between FCM and IC was 12 of 16 (75%). The extraosseous Ewing's sarcoma tumors were 2 diploid and 1 aneuploid by IC. In this study there was no correlation between the DNA ploidy and either the histology, site, or survival.
A Kowal-Vern; J Walloch; P Chou; F Gonzalez-Crussi; J Price; D Potocki; C Herman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  5     ISSN:  0893-3952     ISO Abbreviation:  Mod. Pathol.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-04-09     Completed Date:  1992-04-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  56-60     Citation Subset:  IM    
Loyola University Medical Center, Department of Pathology, Maywood, Illinois.
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MeSH Terms
DNA, Neoplasm / analysis*,  genetics
Flow Cytometry
Head and Neck Neoplasms / genetics*
Pelvic Neoplasms / genetics*
Sarcoma, Ewing's / genetics*
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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