| Flow cytometry study of the role of superoxide anion and hydrogen peroxide in cellular photodestruction with 5-aminolevulinic acid-induced protoporphyrin IX. | |
| | |
MedLine Citation:
|
PMID: 9361128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Flow cytometry was used to investigate the participation of reactive oxygen species, other than singlet oxygen, in the cytotoxic effect of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in vitro in A-431 squamous cell carcinoma (SCC) cells and human skin fibroblasts (HSF). We used propidium iodide to determine cellular cytotoxicity, hydroethidine to measure intracellular superoxide anion (O2-) and dihydrorhodamine 123 to assess intracellular hydrogen peroxide (H2O2) content. Our data support the importance of the incubation time with ALA in the selectivity of PDT with ALA against SCC cells, inducing minimum damage on normal HSF. Photoradiation mortality curves of the response of these cell lines to ALA-induced PpIX photosensitization correlated with the extent of photosensitizer accumulation. Intracellular O2- production correlated with cell death, increasing both in a light dose-dependent fashion in ALA treated cells. This correlation was not observed with H2O2-intracellular production. These results suggest the effectiveness of PDT with ALA in vitro in SCC, the significant participation of O2- in its phototoxic mechanism, and the usefulness of flow cytometry in the study of the cytotoxic effect of ALA-induced PpIX PDT. |
| | |
Authors:
|
Y Gilaberte; D Pereboom; F J Carapeto; J O Alda |
Related Documents
:
|
14695918 - Specificity of coenzyme q inhibition of an aging-related cell surface nadh oxidase (ect... 12855558 - Bcl2 retards g1/s cell cycle transition by regulating intracellular ros. 17482568 - Relationship between reactive oxygen species and heme metabolism during the differentia... 1692968 - Detection of free radical-induced dna damage with bromodeoxyuridine/hoechst flow cytome... 11113198 - Different domains of the essential gtpase cdc42p required for growth and development of... 7733628 - 5-fluorouracil cytotoxicity in human colon ht-29 cells with moderately increased or dec... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Photodermatology, photoimmunology & photomedicine Volume: 13 ISSN: 0905-4383 ISO Abbreviation: Photodermatol Photoimmunol Photomed Publication Date: 1997 Feb-Apr |
Date Detail:
|
Created Date: 1998-01-12 Completed Date: 1998-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9013641 Medline TA: Photodermatol Photoimmunol Photomed Country: DENMARK |
Other Details:
|
Languages: eng Pagination: 43-9 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, Zaragoza Medical School, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aminolevulinic Acid / therapeutic use* Analysis of Variance Carcinoma, Squamous Cell / drug therapy, metabolism Cell Death Cell Survival Coloring Agents / diagnostic use Dose-Response Relationship, Radiation Female Fibroblasts / cytology, drug effects, metabolism, radiation effects Flow Cytometry* Fluorescent Dyes / diagnostic use Humans Hydrogen Peroxide / analysis, metabolism* Oxidants / analysis, metabolism* Phenanthridines / diagnostic use Photochemotherapy* Photosensitizing Agents / therapeutic use* Propidium / diagnostic use Protoporphyrins / therapeutic use* Reactive Oxygen Species / metabolism Rhodamines / diagnostic use Skin / cytology, drug effects, metabolism, radiation effects Superoxides / analysis, metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Coloring Agents; 0/Fluorescent Dyes; 0/Oxidants; 0/Phenanthridines; 0/Photosensitizing Agents; 0/Protoporphyrins; 0/Reactive Oxygen Species; 0/Rhodamines; 106-60-5/Aminolevulinic Acid; 109244-58-8/dihydrorhodamine 123; 11062-77-4/Superoxides; 36015-30-2/Propidium; 38483-26-0/hydroethidine; 553-12-8/protoporphyrin IX; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Microfluorometry using fluorescein diacetate reflects the integrity of the plasma membrane in UVA-ir...
Next Document: Topical or oral administration with an extract of Polypodium leucotomos prevents acute sunburn and p...