Document Detail


Flow cytometry of mitotic cells.
MedLine Citation:
PMID:  9473359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a procedure using flow cytometric measurement of a mitosis-specific antigen that may be used to count mitotic cells and sort them from non-mitotic cells. The procedure may also be used in conjunction with measurement of cellular DNA content and of bromodeoxyuridine incorporation into cellular DNA to assign cells to the G1/G0, S, G2, or M phase of the cell cycle.
Authors:
H J Anderson; G de Jong; I Vincent; M Roberge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  238     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-12     Completed Date:  1998-03-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  498-502     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Affiliation:
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Antibody Specificity
Antigens / analysis
Breast Neoplasms
Carcinoma
Cell Separation
DNA, Neoplasm / analysis
Flow Cytometry / methods*
Humans
Interphase
Mitosis*
Nocodazole / pharmacology
Phosphoproteins / analysis
RNA-Binding Proteins / analysis
Tumor Cells, Cultured
tau Proteins / analysis
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/DNA, Neoplasm; 0/Phosphoproteins; 0/RNA-Binding Proteins; 0/nucleolin; 0/tau Proteins; 31430-18-9/Nocodazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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