| Flow cytometry methods for the study of cell-cycle parameters of planarian stem cells. | |
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MedLine Citation:
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PMID: 19322765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Due to their characteristic inaccessibility and low numbers, little is known about the cell-cycle dynamics of most stem cells in vivo. A powerful, established methodology to study cell-cycle dynamics is flow cytometry, which is used routinely to study the cell-cycle dynamics of proliferating cells in vitro. Its use in heterogeneous mixtures of cells obtained from whole animals, however, is complicated by the relatively low abundance of cycling to non-cycling cells. We report on flow cytometric methods that take advantage of the abundance of proliferating stem cells in the planarian Schmidtea mediterranea. The optimized protocols allow us to measure cell-cycle dynamics and follow BrdU-labeled cells specifically in complex mixtures of cells. These methods expand on the growing toolkit being developed to study stem cell biology in planarians, and open the door to detailed cytometric studies of a collectively totipotent population of adult stem cells in vivo. |
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Authors:
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Hara Kang; Alejandro Sánchez Alvarado |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 238 ISSN: 1058-8388 ISO Abbreviation: Dev. Dyn. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-30 Completed Date: 2009-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 1111-7 Citation Subset: IM |
Affiliation:
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Department of Neurobiology and Anatomy, Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84132, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bromodeoxyuridine / metabolism Cell Cycle* / genetics Cell Differentiation / physiology Cell Proliferation Flow Cytometry / methods* Planarians / cytology* RNA Interference / physiology Stem Cells / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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//Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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