Document Detail

Flow cytometry of mesophyll and bundle sheath chloroplast thylakoids of maize (Zea mays L.).
MedLine Citation:
PMID:  8742167     Owner:  NLM     Status:  MEDLINE    
Chlorophyll fluorescence at short and long wavelengths was used to sort thylakoid membranes of maize, a plant with the C4 dicarboxylic acid pathway of photosynthesis, in a flow cytometer. The method yielded two distinct particle populations that were identified as mesophyll and bundle sheath thylakoids by low-temperature fluorescence spectroscopy and by the pigment ratio of chlorophyll a/b. Mesophyll and bundle sheath thylakoids were essentially pure after sorting by flow cytometry. Fluorescence data and chlorophyll a/b pigment ratios of thylakoids separated by flow cytometry were compared with earlier data of preparations obtained by conventional isolation procedures. Our results indicate that impure mesophyll and bundle sheath membranes were used in most previous investigations. We were unable to detect the major light-harvesting complex of PS II (LHC II) in our pure bundle sheath thylakoids using fluorescence excitation spectroscopy. Therefore, we believe that the previously reported presence of LHC II in bundle sheath chloroplasts of maize can be attributed to mesophyll contamination.
E Pfündel; A Meister
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry     Volume:  23     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-10-10     Completed Date:  1996-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  97-105     Citation Subset:  IM    
Institut für Pflanzengenetik und Kulturpflanzenforschung, Federal Republic of Germany.
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MeSH Terms
Chloroplasts / physiology,  ultrastructure*
Flow Cytometry*
Intracellular Membranes / ultrastructure*
Peas / physiology
Photosynthesis / physiology
Spectrometry, Fluorescence
Zea mays / physiology,  ultrastructure*

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