Document Detail

Flow cytometry of keratinocytes.
MedLine Citation:
PMID:  6188766     Owner:  NLM     Status:  MEDLINE    
A prerequisite for using flow cytometry (FCM) is the availability of isolated single cells. Procedures for separation and isolation of keratinocytes from animals and man are available, and the resulting single cell suspensions have been subjected to FCM measurements. The major advantage of the method is the accuracy and speed with which a variety of cellular constituents can be quantified. FCM of keratinocytes has, hitherto, been mainly confined to measurements of nuclear DNA for estimation of cell-cycle distributions and for ploidy studies. In mouse epidermis, cell-cycle distributions were estimated from sequentially obtained DNA histograms and evaluated with other cell kinetic measurements, resulting in new information about epidermal cell-cycle progression, not achievable by any of the methods alone. The best way, therefore, to increase our knowledge of keratinocyte proliferation, is the combined use of DNA FCM and other cell kinetic methods. DNA FCM has also been applied to healthy and diseased human epidermis, and may add valuable information to the classification of skin disease in selected cases. It is believed that further progress in the characterization of keratinocyte growth and development will depend on parameters other than DNA alone.
O P Clausen
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Publication Detail:
Type:  In Vitro; Journal Article; Review    
Journal Detail:
Title:  Journal of cutaneous pathology     Volume:  10     ISSN:  0303-6987     ISO Abbreviation:  J. Cutan. Pathol.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-06-10     Completed Date:  1983-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0425124     Medline TA:  J Cutan Pathol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  33-51     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell Separation
Circadian Rhythm
DNA / analysis*,  biosynthesis
Epidermis / cytology*,  metabolism
Epithelium / metabolism
Flow Cytometry*
Keratins / metabolism*
Mice, Hairless
RNA / analysis
Skin Diseases / metabolism
Skin Neoplasms / analysis
Staining and Labeling
Reg. No./Substance:
63231-63-0/RNA; 68238-35-7/Keratins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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