| Flow cytometry of keratinocytes. | |
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MedLine Citation:
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PMID: 6188766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A prerequisite for using flow cytometry (FCM) is the availability of isolated single cells. Procedures for separation and isolation of keratinocytes from animals and man are available, and the resulting single cell suspensions have been subjected to FCM measurements. The major advantage of the method is the accuracy and speed with which a variety of cellular constituents can be quantified. FCM of keratinocytes has, hitherto, been mainly confined to measurements of nuclear DNA for estimation of cell-cycle distributions and for ploidy studies. In mouse epidermis, cell-cycle distributions were estimated from sequentially obtained DNA histograms and evaluated with other cell kinetic measurements, resulting in new information about epidermal cell-cycle progression, not achievable by any of the methods alone. The best way, therefore, to increase our knowledge of keratinocyte proliferation, is the combined use of DNA FCM and other cell kinetic methods. DNA FCM has also been applied to healthy and diseased human epidermis, and may add valuable information to the classification of skin disease in selected cases. It is believed that further progress in the characterization of keratinocyte growth and development will depend on parameters other than DNA alone. |
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Authors:
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O P Clausen |
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Publication Detail:
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Type: In Vitro; Journal Article; Review |
Journal Detail:
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Title: Journal of cutaneous pathology Volume: 10 ISSN: 0303-6987 ISO Abbreviation: J. Cutan. Pathol. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-06-10 Completed Date: 1983-06-10 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0425124 Medline TA: J Cutan Pathol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 33-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cell Separation Circadian Rhythm Cricetinae DNA / analysis*, biosynthesis Epidermis / cytology*, metabolism Epithelium / metabolism Flow Cytometry* Humans Keratins / metabolism* Mice Mice, Hairless RNA / analysis Skin Diseases / metabolism Skin Neoplasms / analysis Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA; 68238-35-7/Keratins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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